Freimoser:Recipes:NZY: Difference between revisions
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NZY medium
Composition (for 1L)
- 10g Yeast extract
- 5g NaCl
- 2g Glucose
- 16g NZ-Amine A
Fill up to 1 liter and adjust to pH 7.
Comment
- We use this medium to grow bacteria if purification on amylose resin (with MBP tag) is sought
- The use of NZ-amine A also lactose free (in contrast to bacteriological peptone)
- NZ-amine A is available from Sigma (C0626-250g)