Freimoser:Recipes:DiluentA: Difference between revisions
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===Use=== | |||
Diluent A is used to dilute NEB T4 ligase [http://www.neb.com/nebecomm/products/productM0202.asp] to a final concentration of 1U/ml (400x dilution). | |||
===Composition=== | ===Composition=== | ||
10 mM Tris-HCl | *10 mM Tris-HCl | ||
1 mM Dithiothreitol (DTT) | *1 mM Dithiothreitol (DTT) | ||
0.1 mM EDTA | *0.1 mM EDTA | ||
50% Glycerol | *50% Glycerol | ||
200 mg/ml BSA | *200 mg/ml BSA | ||
50 mM KCl | *50 mM KCl | ||
Adjust pH to 7.4 (25°C) | |||
Revision as of 08:47, 30 October 2006
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Use
Diluent A is used to dilute NEB T4 ligase [1] to a final concentration of 1U/ml (400x dilution).
Composition
- 10 mM Tris-HCl
- 1 mM Dithiothreitol (DTT)
- 0.1 mM EDTA
- 50% Glycerol
- 200 mg/ml BSA
- 50 mM KCl
Adjust pH to 7.4 (25°C)
