Fong:Protocols
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Media and Reagents
- Fong:LB
- Fong:Antibiotics - Antibiotic stock preparation
- Fong:IPTG - IPTG stock
- CTFUD - Rich medium for C. thermocellum. Contact Chris for this protocol.
- Fong:MJ - Minimal medium for C. thermocellum
- Fong:TFU - Medium for T. fusca
Equipment
- Fong:Spectrophotometry and plate reader use
- Fong:HPLC
General techniques
- Fong:Lab Basics - safety, waste/biohazard disposal, dishwashing/autoclaving, storage issues
- Fong:Cryogenic storage
- Fong:Serum bottle anaerobic culture - General methodology for sterile anaerobic bacterial culture
- Fong:Bradford protein assay - Colorimetric assay for protein concentration, useful for monitoring cell growth in the presence of insoluble substrates
- Fong:Electrocompetency
- Fong:Electrotransformation of E. coli
- Fong:Transformation of T. fusca
- Fong:PCR
- Fong:Quantification of free Cellobiose
- Fong:MetModel - Analysis and Utilization of metabolic network modeling.
Nucleic acids
- Fong:Plasmid preparation
- Fong:RNA gel electrophoresis/Denaturing
- Ethanol precipitation of nucleic acids - This consensus protocol provides a great overview. There is a microcentrifuge in the refrigerator in room 435.
- Fong:DNA gel electrophoresis
- Fong:BioBrick assembly