Fong:Protocols: Difference between revisions
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*[[Fong:MJ]] - Minimal medium for ''C. thermocellum'' | *[[Fong:MJ]] - Minimal medium for ''C. thermocellum'' | ||
*[[Fong:TFU]] - Medium for ''T. fusca'' | *[[Fong:TFU]] - Medium for ''T. fusca'' | ||
==Equipment== | |||
*[[Fong:Spectrophotometry]] and plate reader use | |||
*[[Fong:HPLC]] | |||
==General techniques== | ==General techniques== | ||
*[[Fong:Lab Basics]] - safety, waste/biohazard disposal, dishwashing/autoclaving, storage issues | *[[Fong:Lab Basics]] - safety, waste/biohazard disposal, dishwashing/autoclaving, storage issues | ||
*[[Fong:Cryogenic storage]] | *[[Fong:Cryogenic storage]] | ||
*[[Fong:Serum bottle anaerobic culture]] - General methodology for sterile anaerobic bacterial culture | *[[Fong:Serum bottle anaerobic culture]] - General methodology for sterile anaerobic bacterial culture | ||
Revision as of 13:12, 21 July 2009
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Media and Reagents
- Fong:LB
- Fong:Antibiotics - Antibiotic stock preparation
- Fong:IPTG - IPTG stock
- CTFUD - Rich medium for C. thermocellum. Contact Chris for this protocol.
- Fong:MJ - Minimal medium for C. thermocellum
- Fong:TFU - Medium for T. fusca
Equipment
- Fong:Spectrophotometry and plate reader use
- Fong:HPLC
General techniques
- Fong:Lab Basics - safety, waste/biohazard disposal, dishwashing/autoclaving, storage issues
- Fong:Cryogenic storage
- Fong:Serum bottle anaerobic culture - General methodology for sterile anaerobic bacterial culture
- Fong:Bradford protein assay - Colorimetric assay for protein concentration, useful for monitoring cell growth in the presence of insoluble substrates
- Fong:Electrocompetency
- Fong:Electrotransformation of E. coli
- Fong:Transformation of T. fusca
- Fong:PCR
- Fong:Quantification of free Cellobiose
Nucleic acids
- Fong:Plasmid preparation
- Fong:RNA gel electrophoresis/Denaturing
- Ethanol precipitation of nucleic acids - This consensus protocol provides a great overview. There is a microcentrifuge in the refrigerator in room 435.
- Fong:DNA gel electrophoresis
- Fong:BioBrick assembly
