Fong:Protocols: Difference between revisions
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[[Fong:Bradford protein assay]] - Colorimetric assay for protein concentration, useful for monitoring cell growth in the presence of insoluble substrates | [[Fong:Bradford protein assay]] - Colorimetric assay for protein concentration, useful for monitoring cell growth in the presence of insoluble substrates | ||
[[Fong:Electrocompetency]] | |||
[[Fong:Electrotransformation]] of ''E. coli'' | [[Fong:Electrotransformation]] of ''E. coli'' | ||
Revision as of 12:20, 3 June 2009
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Media and Reagents
LB broth / plates
Fong:Antibiotics - Antibiotic stock preparation
CTFUD - Rich medium for C. thermocellum. Contact Chris for this protocol.
Fong:MJ - Minimal medium for C. thermocellum
Fong:TFU - Medium for T. fusca
General techniques
Fong:Lab Basics - safety, waste/biohazard disposal, dishwashing/autoclaving, storage issues
Fong:Spectrophotometry and plate reader use
Fong:Serum bottle anaerobic culture - General methodology for sterile anaerobic bacterial culture
Fong:Bradford protein assay - Colorimetric assay for protein concentration, useful for monitoring cell growth in the presence of insoluble substrates
Fong:Electrotransformation of E. coli
Fong:Transformation of T. fusca
Nucleic acids
Fong:RNA gel electrophoresis/Denaturing
Ethanol precipitation of nucleic acids - This consensus protocol provides a great overview. There is a microcentrifuge in the refrigerator in room 435.
