Fong:Protocols: Difference between revisions
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==General techniques== | ==General techniques== | ||
Lab Basics - safety, waste/biohazard disposal, dishwashing/autoclaving, storage issues | [[Fong:Lab Basics]] - safety, waste/biohazard disposal, dishwashing/autoclaving, storage issues | ||
Spectrophotometry / plate reader | Spectrophotometry / plate reader | ||
Revision as of 12:26, 8 May 2009
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Media and Reagents
Fong:CTFUD - Rich medium for C. thermocellum
Fong:MJ - Minimal medium for C. thermocellum
LB broth / plates
General techniques
Fong:Lab Basics - safety, waste/biohazard disposal, dishwashing/autoclaving, storage issues
Spectrophotometry / plate reader
Fong:Serum bottle anaerobic culture - General methodology for sterile anaerobic bacterial culture
Fong:Bradford protein assay - Colorimetric assay for protein concentration, useful for monitoring cell growth in the presence of insoluble substrates
Transformation
Nucleic acids
Fong:RNA gel electrophoresis/Denaturing
Ethanol precipitation of nucleic acids - This consensus protocol provides a great overview. There is a microcentrifuge in the refrigerator in room 435.
