Fong:LB: Difference between revisions
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GMcArthurIV (talk | contribs) (New page: {{Template:Fong}} Protocol for the preparation of lysogeny broth ==Materials== *Pure water *LB poweder *Agar (if making dishes) ==Procedure== #Weigh out 20g of LB powder and place in a c...) |
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Protocol for the preparation of lysogeny broth
Materials
- Pure water
- LB poweder
- Agar (if making dishes)
Procedure
- Weigh out 20g of LB powder and place in a clean 1L bottle
- Add 18g of agar (only if making semi-solid media in dishes)
- Fill to 1L with pure water
- Autoclave solution for 15 minutes on "liquids cycle"
Pouring dishes
- Add appropriate antibiotic to LB once it has cooled
- Pour liquid media before it starts to set - cover bottom of dish & just a bit more
- Label each dish with LB, antibiotic (include concentration if different from standard), date and initials
- Store in refrigerator within plastic sleeve