Fixing cells: Difference between revisions
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2) For permeabilization, remove Formaldehyde Solution, and incubate cells in Permeabilization Solution for 5 minutes at room temperature. | 2) For permeabilization, remove Formaldehyde Solution, and incubate cells in Permeabilization Solution for 5 minutes at room temperature. | ||
For further information about fixing cells, see [[Immunocytochemistry]]. | |||
Revision as of 10:21, 15 June 2006
Formaldehyde Fixation
- This works well for fixation of mammalian cells in preparation for immunolabeling and microscopy
Reagents
-Fixation Solution: 4% Formaldehyde in PBS
-Permeabilization Solution: 0.1% Triton X-100 in PBS
Protocol
1) For fixation, incubate cells in Formaldehyde Solution for 10-15 minutes at room temperature.
2) For permeabilization, remove Formaldehyde Solution, and incubate cells in Permeabilization Solution for 5 minutes at room temperature.
For further information about fixing cells, see Immunocytochemistry.
