Fixing cells: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 5: | Line 5: | ||
'''Reagents''' | '''Reagents''' | ||
-Fixation Solution: 4% Formaldehyde in PBS | -Fixation Solution: 4% Formaldehyde in PBS | ||
-Permeabilization Solution: 0.1% Triton X-100 in PBS | -Permeabilization Solution: 0.1% Triton X-100 in PBS | ||
Revision as of 23:13, 25 February 2006
Formaldehyde Fixation
- This works well for fixation of mammalian cells in preparation for immunolabeleing and microscopy
Reagents -Fixation Solution: 4% Formaldehyde in PBS
-Permeabilization Solution: 0.1% Triton X-100 in PBS
Protocol
1) For fixation, incubate cells in Formaldehyde Solution for 10-15 minutes at room temperature.
2) For permeabilization, remove Formaldehyde Solution, and incubate cells in Permeabilization Solution for 5 minutes at room temperature.
