Fixing cells

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== '''Formaldehyde Fixation''' ==
== '''Formaldehyde Fixation''' ==
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***This works well for fixation of mammalian cells in preparation for immunolabeling and microscopy
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*This works well for fixation of mammalian cells in preparation for immunolabeling and microscopy
'''Reagents'''
'''Reagents'''

Revision as of 15:17, 14 June 2006

Formaldehyde Fixation

  • This works well for fixation of mammalian cells in preparation for immunolabeling and microscopy

Reagents

-Fixation Solution: 4% Formaldehyde in PBS

-Permeabilization Solution: 0.1% Triton X-100 in PBS

Protocol

1) For fixation, incubate cells in Formaldehyde Solution for 10-15 minutes at room temperature.

2) For permeabilization, remove Formaldehyde Solution, and incubate cells in Permeabilization Solution for 5 minutes at room temperature.

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