Fixing cells

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== '''Formaldehyde Fixation''' ==
== '''Formaldehyde Fixation''' ==
***This works well for fixation of mammalian cells in preparation for immunolabeleing and microscopy
***This works well for fixation of mammalian cells in preparation for immunolabeleing and microscopy
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1)  Incubate cells in ~4% Formaldehyde solution (in PBS) for 10-15 minutes at room temperature.
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'''Reagents'''
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-Fixation Solution: 4% Formaldehyde in PBS
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-Permeabilization Solution: 0.1% Triton X-100 in PBS
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'''Protocol'''
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1) For fixation, incubate cells in Formaldehyde Solution for 10-15 minutes at room temperature.
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2)  For permeabilization of cells, remove formaldehyde solution, and incubate cells in 0.1% Triton X-100 (in PBS) for 5 minutes at room temperature.  There is no need to wash between fixation and permeabilization steps.
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2)  For permeabilization, remove Formaldehyde Solution, and incubate cells in Permeabilization Solution for 5 minutes at room temperature.

Revision as of 02:12, 26 February 2006

Formaldehyde Fixation

      • This works well for fixation of mammalian cells in preparation for immunolabeleing and microscopy

Reagents -Fixation Solution: 4% Formaldehyde in PBS -Permeabilization Solution: 0.1% Triton X-100 in PBS

Protocol

1) For fixation, incubate cells in Formaldehyde Solution for 10-15 minutes at room temperature.

2) For permeabilization, remove Formaldehyde Solution, and incubate cells in Permeabilization Solution for 5 minutes at room temperature.

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