Fixing cells
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'''Formaldehyde Fixation''' | '''Formaldehyde Fixation''' | ||
| - | ***This works well for fixation of mammalian cells in preparation for immunolabeleing and microscopy**** | + | ****This works well for fixation of mammalian cells in preparation for immunolabeleing and microscopy**** |
1) Incubate cells in ~4% Formaldehyde solution (in PBS) for 10-15 minutes at room temperature. | 1) Incubate cells in ~4% Formaldehyde solution (in PBS) for 10-15 minutes at room temperature. | ||
2) For permeabilization of cells, remove formaldehyde solution, and incubate cells in 0.1% Triton X-100 (in PBS) for 5 minutes at room temperature. There is no need for washing between fixation and permeabilization steps. | 2) For permeabilization of cells, remove formaldehyde solution, and incubate cells in 0.1% Triton X-100 (in PBS) for 5 minutes at room temperature. There is no need for washing between fixation and permeabilization steps. | ||
Revision as of 02:02, 26 February 2006
Formaldehyde Fixation
- This works well for fixation of mammalian cells in preparation for immunolabeleing and microscopy****
1) Incubate cells in ~4% Formaldehyde solution (in PBS) for 10-15 minutes at room temperature.
2) For permeabilization of cells, remove formaldehyde solution, and incubate cells in 0.1% Triton X-100 (in PBS) for 5 minutes at room temperature. There is no need for washing between fixation and permeabilization steps.
