http://openwetware.org/index.php?title=Ficoll&feed=atom&action=history 2013-05-19T05:51:53Z Revision history for this page on the wiki MediaWiki 1.13.2 http://openwetware.org/index.php?title=Ficoll&diff=383894&oldid=prev 2010-01-21T14:41:40Z

<p>categories</p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 14:41, 21 January 2010</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 17:</td> <td colspan="2" class="diff-lineno">Line 17:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* [http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Product_Information_Sheet/f4375pis.Par.0001.File.tmp/f4375pis.pdf Ficoll 400 datasheet at Sigma-Aldrich]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* [http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Product_Information_Sheet/f4375pis.Par.0001.File.tmp/f4375pis.pdf Ficoll 400 datasheet at Sigma-Aldrich]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* [http://www4.gelifesciences.com/aptrix/upp01077.nsf/Content/cellpreparation_home~products_cell~density_gradient_media~ficoll_pm400?OpenDocument&amp;moduleid=167163 Ficoll 400 page at GE Healthcare]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* [http://www4.gelifesciences.com/aptrix/upp01077.nsf/Content/cellpreparation_home~products_cell~density_gradient_media~ficoll_pm400?OpenDocument&amp;moduleid=167163 Ficoll 400 page at GE Healthcare]</div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[Category:Material]]</ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[Category:Chemical]]</ins></div></td></tr> <!-- diff generator: internal 2013-05-19 05:51:53 --> </table>

Jakob Suckale http://openwetware.org/index.php?title=Ficoll&diff=383893&oldid=prev 2010-01-21T14:40:57Z

<p>see also</p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 14:40, 21 January 2010</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Ficoll'''™ is a sucrose polymer commonly used to adjust the density and viscosity of solutions in the lab. It is employed in density gradients and loading buffers.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Ficoll'''™ is a sucrose polymer commonly used to adjust the density and viscosity of solutions in the lab. It is employed in density gradients and loading buffers.</div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">== Use ==</ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">One of the most common applications for Ficoll is as a density gradient for the isolation of eukaryotic cells, organelles, bacterial cells, and pancreatic islets.&nbsp; </ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Properties ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Properties ==</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Ficoll is an uncharged, highly branched polymer formed by the co-polymerisation of sucrose and epichlorohydrin. Due to its many hydroxyl groups, Ficoll is highly soluble in water. Densities of up to 1.2 g/ml can be attained.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Ficoll is an uncharged, highly branched polymer formed by the co-polymerisation of sucrose and epichlorohydrin. Due to its many hydroxyl groups, Ficoll is highly soluble in water. Densities of up to 1.2 g/ml can be attained.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>== <del class="diffchange diffchange-inline">Use </del>==</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>== <ins class="diffchange diffchange-inline">Stability &amp; storage </ins>== &nbsp;</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">One of the most common applications for </del>Ficoll <del class="diffchange diffchange-inline">is as a density gradient for </del>the <del class="diffchange diffchange-inline">isolation of eukaryotic cells</del>, <del class="diffchange diffchange-inline">organelles</del>, <del class="diffchange diffchange-inline">bacterial cells</del>, and <del class="diffchange diffchange-inline">pancreatic islets</del>. <del class="diffchange diffchange-inline"> </del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Since </ins>Ficoll <ins class="diffchange diffchange-inline">does not contain any ionized groups, </ins>the <ins class="diffchange diffchange-inline">molecule does not readily react under physiological conditions. It is stable in alkaline and neutral solutions</ins>, <ins class="diffchange diffchange-inline">but is rapidly hydrolysed in solution at pH 3</ins>, &nbsp;</div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">especially at high temperature. However</ins>, <ins class="diffchange diffchange-inline">Ficoll can be autoclaving at 110ºC for 30 minutes in neutral solutions. </ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Strong oxidizing and reducing agents should be avoided. Shipping </ins>and <ins class="diffchange diffchange-inline">storage are at ambient temperatures</ins>. &nbsp;</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== See also ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== See also ==</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* [http://en.wikipedia.org/wiki/Ficoll Ficoll at the Wikipedia]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* [http://en.wikipedia.org/wiki/Ficoll Ficoll at the Wikipedia]</div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">* [http://www.med.upenn.edu/bmcrc/immune/Ficcoll_info.pdf Ficoll 70 and 400 datasheet from Amersham at UPenn]</ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">* [http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Product_Information_Sheet/f4375pis.Par.0001.File.tmp/f4375pis.pdf Ficoll 400 datasheet at Sigma-Aldrich]</ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">* [http://www4.gelifesciences.com/aptrix/upp01077.nsf/Content/cellpreparation_home~products_cell~density_gradient_media~ficoll_pm400?OpenDocument&amp;moduleid=167163 Ficoll 400 page at GE Healthcare]</ins></div></td></tr> <!-- diff generator: internal 2013-05-19 05:51:53 --> </table>

Jakob Suckale http://openwetware.org/index.php?title=Ficoll&diff=383891&oldid=prev 2010-01-21T14:35:18Z

<p>properties &amp; use</p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 14:35, 21 January 2010</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>'''Ficoll'''<del class="diffchange diffchange-inline">&lt;sup&gt;TM&lt;/sup&gt; </del>is <del class="diffchange diffchange-inline">an uncharged, highly branched </del>polymer <del class="diffchange diffchange-inline">formed by </del>the <del class="diffchange diffchange-inline">co-polymerisation </del>of <del class="diffchange diffchange-inline">sucrose </del>and <del class="diffchange diffchange-inline">epichlorohydrin</del>. <del class="diffchange diffchange-inline"> </del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>'''Ficoll'''<ins class="diffchange diffchange-inline">™ </ins>is <ins class="diffchange diffchange-inline">a sucrose </ins>polymer <ins class="diffchange diffchange-inline">commonly used to adjust </ins>the <ins class="diffchange diffchange-inline">density and viscosity </ins>of <ins class="diffchange diffchange-inline">solutions in the lab. It is employed in density gradients </ins>and <ins class="diffchange diffchange-inline">loading buffers</ins>.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>http://en.wikipedia.org/wiki/Ficoll</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">== Properties ==</ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Ficoll is an uncharged, highly branched polymer formed by the co-polymerisation of sucrose and epichlorohydrin. Due to its many hydroxyl groups, Ficoll is highly soluble in water. Densities of up to 1.2 g/ml can be attained.</ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>&#160;</div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">== Use ==</ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">One of the most common applications for Ficoll is as a density gradient for the isolation of eukaryotic cells, organelles, bacterial cells, and pancreatic islets.&nbsp; </ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>&#160;</div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">== See also ==</ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">* [</ins>http://en.wikipedia.org/wiki/Ficoll <ins class="diffchange diffchange-inline">Ficoll at the Wikipedia]</ins></div></td></tr> <!-- diff generator: internal 2013-05-19 05:51:53 --> </table>

Jakob Suckale http://openwetware.org/index.php?title=Ficoll&diff=383890&oldid=prev 2010-01-21T14:22:30Z

<p>*** page created ***</p> <p><b>New page</b></p><div>'''Ficoll'''&lt;sup&gt;TM&lt;/sup&gt; is an uncharged, highly branched polymer formed by the co-polymerisation of sucrose and epichlorohydrin. <br /> <br /> http://en.wikipedia.org/wiki/Ficoll</div>

Jakob Suckale