FCCT Biochemistry Lab:M9 medium/minimal
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We used to prepare the plain M9 medium as follows:
| 900 ml sterile water |
| 100 ml M9 salts stock solution |
| 1 ml autoclaved 1 M MgSO4 |
| 0.1 ml autoclaved 1 M CaCl2 |
M9 salts stock solution (10x) is:
| 60 g Na2HPO4 x 7 H2O |
| 30 g KH2PO4 |
| 5 g NaCl |
| 10 g NH4Cl |
| add water to 1000 ml and autoclave |
Notes
Note 1: salts that are added last often tend to precipitate. I didn't bother about it too much as obviously it didn't influence bacterial growth. There might be a way to avoid precipitation (eventually adding magnesium and calcium first) but I didn't investigate this in detail.
Note 2: The M9 stock solution here is essentially as in the M9 salts recipe just that the concentration of Na-phosphate is not exactly the same - see the Talk section therein.
