Expression Analysis Services
From OpenWetWare
We offer a Expression Analysis Service utilizing the 3 Plaforms.
Key highlights of the service:
Please see below for further details about the service.
Sample Submission Guidelines
- Customer should submit atleast 5 ug of Total RNA or 2 ug of Samples enriched for miRNA.
- RNA concentration and quality should be quantified.
- Sample submission forms should be submitted along or before the samples are submitted. The form is available here.
mRNA Detection Platform
- Affymetrix.
- Agilent
Protocol
Data Extraction
- Microarray images are scanned for a balanced view and saved in TIFF formats.
- Numerical intensities are extracted for each probes
- The results are organized which includes TIFF image files and intensity data files, which is made available to the customer in a CD.
Analysis
- We would do the image analysis and provide you with the raw intensities for all the platforms.
- Certain downstream data processing is done based on the platform, as part of the service:
- Affymetrix data is scaled to a average intensity of 150 per chip.
- Agilent and other 2 color channel data is normalized within array.
Additional data analysis help may be available on a case by case basis.
Pricing
Prices for sample processing do not include cost of arrays
Affymetrix
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Agilent
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