Escherichia coli RNA polymerase - Revision history

Reshma P. Shetty at 14:22, 24 May 2007

Reshma P. Shetty — Thu, 24 May 2007 14:22:07 GMT

←Older revision		Revision as of 14:22, 24 May 2007
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	*1μg T7 D111 DNA as template		*1μg T7 D111 DNA as template

-	[[Category:Escherichia coli]]	+	[[Category:Escherichia coli]] [[Category:Material]] [[Category:Enzyme]] [[Category:Polymerase]] [[Category:RNA]]

Reshma P. Shetty at 13:30, 19 March 2007

Reshma P. Shetty — Mon, 19 Mar 2007 13:30:27 GMT

←Older revision		Revision as of 13:30, 19 March 2007
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	*0.5 mM NTPs		*0.5 mM NTPs
	*1μg T7 D111 DNA as template		*1μg T7 D111 DNA as template
		+
		+	[[Category:Escherichia coli]]

Reshma P. Shetty at 18:49, 5 December 2006

Reshma P. Shetty — Tue, 05 Dec 2006 18:49:03 GMT

New page

[http://www.epibio.com Epicentre] is the only commercial source for ''Escherichia coli'' RNA polymerase.

==Materials==
*[http://www.epibio.com/item.asp?id=275 E. coli RNA Polymerase Core Enzyme and Sigma-Saturated Holoenzyme from Epicentre]

==Reaction conditions==
===For core enzyme===
from [http://www.epibio.com/pdftechlit/015pl096.pdf Epicentre]

===For sigma saturated holoenzyme===
from [http://www.epibio.com/pdftechlit/014pl026.pdf Epicentre]

====Functional test====
50 μL reaction
*1X ''E. coli'' RNA polymerase transcription buffer
**0.04 M Tris-HCl (pH 7.5)
**0.15 M KCl
**10 mM MgCl<sub>2</sub>
**0.01% Triton® X-100
*2mM DTT
*0.25mM NTPs
*1μg T7 D111 DNA as template

====Assay test====
50 μL reaction
*1X ''E. coli'' RNA polymerase transcription buffer
**0.04 M Tris-HCl (pH 7.5)
**0.15 M KCl
**10 mM MgCl<sub>2</sub>
**0.01% Triton® X-100
*10 mM DTT
*0.5 mM NTPs
*1μg T7 D111 DNA as template