Escherichia coli RNA polymerase
From OpenWetWare
(Difference between revisions)
Current revision (10:22, 24 May 2007) (view source) |
|||
| Line 33: | Line 33: | ||
*1μg T7 D111 DNA as template | *1μg T7 D111 DNA as template | ||
| - | [[Category:Escherichia coli]] | + | [[Category:Escherichia coli]] [[Category:Material]] [[Category:Enzyme]] [[Category:Polymerase]] [[Category:RNA]] |
Current revision
Epicentre is the only commercial source for Escherichia coli RNA polymerase.
Contents |
Materials
Reaction conditions
For core enzyme
from Epicentre
For sigma saturated holoenzyme
from Epicentre
Functional test
50 μL reaction
- 1X E. coli RNA polymerase transcription buffer
- 0.04 M Tris-HCl (pH 7.5)
- 0.15 M KCl
- 10 mM MgCl2
- 0.01% Triton® X-100
- 2mM DTT
- 0.25mM NTPs
- 1μg T7 D111 DNA as template
Assay test
50 μL reaction
- 1X E. coli RNA polymerase transcription buffer
- 0.04 M Tris-HCl (pH 7.5)
- 0.15 M KCl
- 10 mM MgCl2
- 0.01% Triton® X-100
- 10 mM DTT
- 0.5 mM NTPs
- 1μg T7 D111 DNA as template
