Escherichia coli/Vectors
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Contents |
Common vectors
| Plasmid | Replicon | Copy number |
| pBR322[1] and its derivatives | pMB1 | 15-20 |
| pUC vectors | pMB1 | 500-700 |
| pACYC and its derivatives | p15A[2] | 10-12 |
| pSC101 and its derivatives | pSC101[3] | 5 |
| ColE1 | ColE1[4, 5] | 15-20 |
Replicon compatibility
| Incompatibility group | Negative control element | Comment |
| colE1[4, 5], pMB1 | RNAI | controls processing of pre-RNAII into primer. pUC is derived from pBR322 (a single mutation in the pBR322 Primer RNA and deletion of the rop gene) which is derived from a pMB1 replicon, and cannot co-reside with the colE1 incompatibility group. |
| IncFII, pT181 | RNA | controls synthesis of RepA protein |
| P1, F, R6K, pSC101[3], p15A[2] | iterons | sequesters RepA protein |
Replication origins in different incompatibility groups are compatible. Replication origins in the same incompatibility group are not.
Some sets of vectors with compatible origins are available as a part of the Novagen Duet system. (from TK)
Individual vector links
Note: searching for cloning vector <insert vector name> when looking for vector sequences in NCBI Entrez Nucleotide search. It helps to cut down on the number of hits.
References
- Bolivar F, Rodriguez RL, Greene PJ, Betlach MC, Heyneker HL, Boyer HW, Crosa JH, and Falkow S. . pmid:344137.
vector pBR322 - Chang AC and Cohen SN. . pmid:149110.
p15A origin - Cohen SN and Chang AC. . pmid:334752.
pSC101 origin - Bazaral M and Helinski DR. . pmid:4939624.
original discovery of ColE1 plasmid - Hershfield V, Boyer HW, Yanofsky C, Lovett MA, and Helinski DR. . pmid:4610576.
use of ColEI as a vector - Joseph Sambrook, David W. Russell. Molecular cloning. Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory, 2001. isbn:0-87969-577-3.
- Anderson ES. . pmid:4879515.
Discusses transferable drug resistance in Escherichia coli (i.e. plasmids with resistance markers) - Cohen SN and Chang AC. . pmid:4576014.
Discusses generation of plasmids by sharing DNA, their transformation and replication. - Cohen SN, Chang AC, Boyer HW, and Helling RB. . pmid:4594039.
Discusses use of vector to propagate other DNA fragments
External links
- Novagen pET vector table
- table with links to properties/sequences of pET vectors
- note: slow to load
