Escherichia coli/Vectors - Revision history

Etienne Robillard: /* Stringent vs. relaxed replication */

Etienne Robillard — Tue, 11 Dec 2012 13:36:58 GMT

Stringent vs. relaxed replication

←Older revision		Revision as of 13:36, 11 December 2012
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	===Stringent vs. relaxed replication===		===Stringent vs. relaxed replication===
-	Plasmid replication control is usually controlled by balancing the levels of a positive and a negative regulator of replication. For some plasmids (pMB1/colE1 replicons) the positive regulator is an RNA and in others (e.g. pSC101) it is a protein. Plasmids with a protein positive regulator will not replicate in the absence of protein production - ''stringent control'' (although not the same as the stringent response due to a shortage of loaded tRNAs). Plasmids with an RNA positive regulator will continue to replicate in the ~~abscence~~ of protein production. This is termed ''relaxed control''. High yields of plasmid may be obtained by halting protein production (via chloroamphenicol) when the culture reaches a high density and then continuing incubation for a number of hours. This might be of practical relevance when prepping the 1 and 3 series of Synthetic Biology plasmids.--[[User:Bcanton\|BC]] 19:05, 3 Sep 2005 (EDT)	+	Plasmid replication control is usually controlled by balancing the levels of a positive and a negative regulator of replication. For some plasmids (pMB1/colE1 replicons) the positive regulator is an RNA and in others (e.g. pSC101) it is a protein. Plasmids with a protein positive regulator will not replicate in the absence of protein production - ''stringent control'' (although not the same as the stringent response due to a shortage of loaded tRNAs). Plasmids with an RNA positive regulator will continue to replicate in the absence of protein production. This is termed ''relaxed control''. High yields of plasmid may be obtained by halting protein production (via chloroamphenicol) when the culture reaches a high density and then continuing incubation for a number of hours. This might be of practical relevance when prepping the 1 and 3 series of Synthetic Biology plasmids.--[[User:Bcanton\|BC]] 19:05, 3 Sep 2005 (EDT)

	===Nomenclature===		===Nomenclature===

Etienne Robillard: /* Stringent vs. relaxed replication */

Etienne Robillard — Tue, 11 Dec 2012 13:36:10 GMT

Stringent vs. relaxed replication

←Older revision		Revision as of 13:36, 11 December 2012
Line 2:		Line 2:

	===Stringent vs. relaxed replication===		===Stringent vs. relaxed replication===
-	Plasmid replication control is usually controlled by balancing the levels of a positive and a negative regulator of replication. For some plasmids (pMB1/colE1 replicons) the positive regulator is an RNA and in others (e.g. pSC101) it is a protein. Plasmids with a protein positive regulator will not replicate in the ~~abscence~~ of protein production - ''stringent control'' (although not the same as the stringent response due to a shortage of loaded tRNAs). Plasmids with an RNA positive regulator will continue to replicate in the abscence of protein production. This is termed ''relaxed control''. High yields of plasmid may be obtained by halting protein production (via chloroamphenicol) when the culture reaches a high density and then continuing incubation for a number of hours. This might be of practical relevance when prepping the 1 and 3 series of Synthetic Biology plasmids.--[[User:Bcanton\|BC]] 19:05, 3 Sep 2005 (EDT)	+	Plasmid replication control is usually controlled by balancing the levels of a positive and a negative regulator of replication. For some plasmids (pMB1/colE1 replicons) the positive regulator is an RNA and in others (e.g. pSC101) it is a protein. Plasmids with a protein positive regulator will not replicate in the absence of protein production - ''stringent control'' (although not the same as the stringent response due to a shortage of loaded tRNAs). Plasmids with an RNA positive regulator will continue to replicate in the abscence of protein production. This is termed ''relaxed control''. High yields of plasmid may be obtained by halting protein production (via chloroamphenicol) when the culture reaches a high density and then continuing incubation for a number of hours. This might be of practical relevance when prepping the 1 and 3 series of Synthetic Biology plasmids.--[[User:Bcanton\|BC]] 19:05, 3 Sep 2005 (EDT)

	===Nomenclature===		===Nomenclature===

Christopher C Vanlang: /* See also */

Christopher C Vanlang — Sun, 01 Jul 2012 10:51:14 GMT

Reshma P. Shetty at 15:24, 25 July 2008

Reshma P. Shetty — Fri, 25 Jul 2008 15:24:14 GMT

←Older revision		Revision as of 15:24, 25 July 2008
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	\|-		\|-
	\|pACYC and its derivatives		\|pACYC and its derivatives
-	\|p15A<cite>Chang-JBacteriol-1978</cite>	+	\|[[p15A]]<cite>Chang-JBacteriol-1978</cite>
	\|10-12		\|10-12
	\|-		\|-
-	\|pSC101 and its derivatives	+	\|[[pSC101]] and its derivatives
	\|pSC101<cite>Cohen-JBacteriol-1977</cite>		\|pSC101<cite>Cohen-JBacteriol-1977</cite>
	\|5		\|5
	\|-		\|-
-	\|ColE1	+	\|[[ColE1]]
	\|ColE1<cite>Bazaral-J-Mol-Biol-1968,Hershfield-Proc-Natl-Acad-Sci-USA-1974</cite>		\|ColE1<cite>Bazaral-J-Mol-Biol-1968,Hershfield-Proc-Natl-Acad-Sci-USA-1974</cite>
	\|15-20		\|15-20

Gwen: /* External links */

Gwen — Tue, 08 Apr 2008 06:08:01 GMT

External links

←Older revision		Revision as of 06:08, 8 April 2008
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	**table with links to properties/sequences of pET vectors		**table with links to properties/sequences of pET vectors
	**note: slow to load		**note: slow to load
		+
		+	*[[Ecoliwiki:Category:Plasmids\|Plasmids at EcoliWiki.org]]
		+	**EcoliWiki.org has entered many plasmids that are available for annotation. Included are plasmid maps, features and sequences.

	==See also==		==See also==

Reshma P. Shetty: /* Individual vector links */

Reshma P. Shetty — Tue, 12 Feb 2008 20:46:28 GMT

Individual vector links

←Older revision		Revision as of 20:46, 12 February 2008
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	*[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=42704 pSC101 replication origin]		*[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=42704 pSC101 replication origin]
	*[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=208958 pBR322]		*[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=208958 pBR322]
		+	*[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=58255 pACYC184]

	==References==		==References==

Reshma P. Shetty: /* Individual vector links */

Reshma P. Shetty — Tue, 12 Feb 2008 01:01:24 GMT

Individual vector links

←Older revision		Revision as of 01:01, 12 February 2008
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	*pSCANS [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=Nucleotide&cmd=Retrieve&list_uids=6689911&dopt=GenBank genbank] [http://genome.bnl.gov/Vectors/pscans.php vector info] [http://www.genome.bnl.gov/Protocols/pscans.shtml cookbook]		*pSCANS [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=Nucleotide&cmd=Retrieve&list_uids=6689911&dopt=GenBank genbank] [http://genome.bnl.gov/Vectors/pscans.php vector info] [http://www.genome.bnl.gov/Protocols/pscans.shtml cookbook]
	*[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=42704 pSC101 replication origin]		*[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=42704 pSC101 replication origin]
		+	*[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=208958 pBR322]

	==References==		==References==

Reshma P. Shetty at 23:10, 20 December 2007

Reshma P. Shetty — Thu, 20 Dec 2007 23:10:58 GMT

←Older revision		Revision as of 23:10, 20 December 2007
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	// Discusses transferable drug resistance in ''Escherichia coli'' (i.e. plasmids with resistance markers)		// Discusses transferable drug resistance in ''Escherichia coli'' (i.e. plasmids with resistance markers)
	#Cohen-Proc-Natl-Acad-Sci-USA-1973a pmid=4576014		#Cohen-Proc-Natl-Acad-Sci-USA-1973a pmid=4576014
-	// Discusses generation of plasmids by ~~sharing~~ DNA, their transformation and replication.	+	// Discusses generation of plasmids by shearing DNA, their transformation and replication.
	#Cohen-Proc-Natl-Acad-Sci-USA-1973b pmid=4594039		#Cohen-Proc-Natl-Acad-Sci-USA-1973b pmid=4594039
	// Discusses use of vector to propagate other DNA fragments		// Discusses use of vector to propagate other DNA fragments

Reshma P. Shetty at 21:57, 20 December 2007

Reshma P. Shetty — Thu, 20 Dec 2007 21:57:58 GMT

←Older revision		Revision as of 21:57, 20 December 2007
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	===Stringent vs. relaxed replication===		===Stringent vs. relaxed replication===
	Plasmid replication control is usually controlled by balancing the levels of a positive and a negative regulator of replication. For some plasmids (pMB1/colE1 replicons) the positive regulator is an RNA and in others (e.g. pSC101) it is a protein. Plasmids with a protein positive regulator will not replicate in the abscence of protein production - ''stringent control'' (although not the same as the stringent response due to a shortage of loaded tRNAs). Plasmids with an RNA positive regulator will continue to replicate in the abscence of protein production. This is termed ''relaxed control''. High yields of plasmid may be obtained by halting protein production (via chloroamphenicol) when the culture reaches a high density and then continuing incubation for a number of hours. This might be of practical relevance when prepping the 1 and 3 series of Synthetic Biology plasmids.--[[User:Bcanton\|BC]] 19:05, 3 Sep 2005 (EDT)		Plasmid replication control is usually controlled by balancing the levels of a positive and a negative regulator of replication. For some plasmids (pMB1/colE1 replicons) the positive regulator is an RNA and in others (e.g. pSC101) it is a protein. Plasmids with a protein positive regulator will not replicate in the abscence of protein production - ''stringent control'' (although not the same as the stringent response due to a shortage of loaded tRNAs). Plasmids with an RNA positive regulator will continue to replicate in the abscence of protein production. This is termed ''relaxed control''. High yields of plasmid may be obtained by halting protein production (via chloroamphenicol) when the culture reaches a high density and then continuing incubation for a number of hours. This might be of practical relevance when prepping the 1 and 3 series of Synthetic Biology plasmids.--[[User:Bcanton\|BC]] 19:05, 3 Sep 2005 (EDT)
		+
		+	===Nomenclature===
		+	<biblio>
		+	#Novick-BacteriolRev-1976 pmid=1267736
		+	</biblio>

	==Common vectors==		==Common vectors==

Reshma P. Shetty at 21:56, 20 December 2007

Reshma P. Shetty — Thu, 20 Dec 2007 21:56:50 GMT

←Older revision		Revision as of 21:56, 20 December 2007
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		+	==General information==
		+
		+	===Stringent vs. relaxed replication===
		+	Plasmid replication control is usually controlled by balancing the levels of a positive and a negative regulator of replication. For some plasmids (pMB1/colE1 replicons) the positive regulator is an RNA and in others (e.g. pSC101) it is a protein. Plasmids with a protein positive regulator will not replicate in the abscence of protein production - ''stringent control'' (although not the same as the stringent response due to a shortage of loaded tRNAs). Plasmids with an RNA positive regulator will continue to replicate in the abscence of protein production. This is termed ''relaxed control''. High yields of plasmid may be obtained by halting protein production (via chloroamphenicol) when the culture reaches a high density and then continuing incubation for a number of hours. This might be of practical relevance when prepping the 1 and 3 series of Synthetic Biology plasmids.--[[User:Bcanton\|BC]] 19:05, 3 Sep 2005 (EDT)
		+
	==Common vectors==		==Common vectors==
	{\|border="1"		{\|border="1"

←Older revision		Revision as of 10:51, 1 July 2012
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	*[[Vectors]]		*[[Vectors]]

-	[[Category:Escherichia coli]] [[Category:DNA]]	+	[[Category:Escherichia coli]] [[Category:DNA]] [[Category:Vectors]]