Escherichia coli/Vectors

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(New page: ==Common vectors== {|border="1" |- |Plasmid |Replicon |Copy number |- |pBR322<cite>Bolivar-Gene-1977</cite> and its derivatives |pMB1 |15-20 |- |pUC vectors |pMB1 |500-700 |- |pACYC and it...)
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*[[Synthetic Biology:Vectors | Synthetic Biology vectors]]
*[[Synthetic Biology:Vectors | Synthetic Biology vectors]]
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[[Category:Escherichia coli]] [[Category:DNA]]

Revision as of 16:55, 20 December 2007

Contents

Common vectors

Plasmid Replicon Copy number
pBR322[1] and its derivatives pMB1 15-20
pUC vectors pMB1 500-700
pACYC and its derivatives p15A[2] 10-12
pSC101 and its derivatives pSC101[3] 5
ColE1 ColE1[4, 5] 15-20

Replicon compatibility

Incompatibility group Negative control element Comment
colE1[4, 5], pMB1 RNAI controls processing of pre-RNAII into primer. pUC is derived from pBR322 (a single mutation in the pBR322 Primer RNA and deletion of the rop gene) which is derived from a pMB1 replicon, and cannot co-reside with the colE1 incompatibility group.
IncFII, pT181 RNA controls synthesis of RepA protein
P1, F, R6K, pSC101[3], p15A[2] iterons sequesters RepA protein

Replication origins in different incompatibility groups are compatible. Replication origins in the same incompatibility group are not.

Some sets of vectors with compatible origins are available as a part of the Novagen Duet system. (from TK)

Individual vector links

Note: searching for cloning vector <insert vector name> when looking for vector sequences in NCBI Entrez Nucleotide search. It helps to cut down on the number of hits.

References

  1. Bolivar F, Rodriguez RL, Greene PJ, Betlach MC, Heyneker HL, Boyer HW, Crosa JH, and Falkow S. . pmid:344137. PubMed HubMed [Bolivar-Gene-1977]
    vector pBR322

  2. Chang AC and Cohen SN. . pmid:149110. PubMed HubMed [Chang-JBacteriol-1978]
    p15A origin

  3. Cohen SN and Chang AC. . pmid:334752. PubMed HubMed [Cohen-JBacteriol-1977]
    pSC101 origin

  4. Bazaral M and Helinski DR. . pmid:4939624. PubMed HubMed [Bazaral-J-Mol-Biol-1968]
    original discovery of ColE1 plasmid

  5. Hershfield V, Boyer HW, Yanofsky C, Lovett MA, and Helinski DR. . pmid:4610576. PubMed HubMed [Hershfield-Proc-Natl-Acad-Sci-USA-1974]
    use of ColEI as a vector

  6. isbn:0-87969-577-3. [MolecularCloning]
  7. Anderson ES. . pmid:4879515. PubMed HubMed [Anderson-Annu-Rev-Microbiol-1968]
    Discusses transferable drug resistance in Escherichia coli (i.e. plasmids with resistance markers)

  8. Cohen SN and Chang AC. . pmid:4576014. PubMed HubMed [Cohen-Proc-Natl-Acad-Sci-USA-1973a]
    Discusses generation of plasmids by sharing DNA, their transformation and replication.

  9. Cohen SN, Chang AC, Boyer HW, and Helling RB. . pmid:4594039. PubMed HubMed [Cohen-Proc-Natl-Acad-Sci-USA-1973b]
    Discusses use of vector to propagate other DNA fragments

All Medline abstracts: PubMed HubMed

External links

See also

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