Escherichia coli/Vectors: Difference between revisions
(New page: ==Common vectors== {|border="1" |- |Plasmid |Replicon |Copy number |- |pBR322<cite>Bolivar-Gene-1977</cite> and its derivatives |pMB1 |15-20 |- |pUC vectors |pMB1 |500-700 |- |pACYC and it...) |
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*[[Synthetic Biology:Vectors | Synthetic Biology vectors]] | *[[Synthetic Biology:Vectors | Synthetic Biology vectors]] | ||
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[[Category:Escherichia coli]] [[Category:DNA]] |
Revision as of 14:55, 20 December 2007
Common vectors
Plasmid | Replicon | Copy number |
pBR322[1] and its derivatives | pMB1 | 15-20 |
pUC vectors | pMB1 | 500-700 |
pACYC and its derivatives | p15A[2] | 10-12 |
pSC101 and its derivatives | pSC101[3] | 5 |
ColE1 | ColE1[4, 5] | 15-20 |
Replicon compatibility
Incompatibility group | Negative control element | Comment |
colE1[4, 5], pMB1 | RNAI | controls processing of pre-RNAII into primer. pUC is derived from pBR322 (a single mutation in the pBR322 Primer RNA and deletion of the rop gene) which is derived from a pMB1 replicon, and cannot co-reside with the colE1 incompatibility group. |
IncFII, pT181 | RNA | controls synthesis of RepA protein |
P1, F, R6K, pSC101[3], p15A[2] | iterons | sequesters RepA protein |
Replication origins in different incompatibility groups are compatible. Replication origins in the same incompatibility group are not.
Some sets of vectors with compatible origins are available as a part of the Novagen Duet system. (from TK)
Individual vector links
Note: searching for cloning vector <insert vector name> when looking for vector sequences in NCBI Entrez Nucleotide search. It helps to cut down on the number of hits.
References
- Bolivar F, Rodriguez RL, Greene PJ, Betlach MC, Heyneker HL, Boyer HW, Crosa JH, and Falkow S. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene. 1977;2(2):95-113.
vector pBR322
- Chang AC and Cohen SN. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J Bacteriol. 1978 Jun;134(3):1141-56. DOI:10.1128/jb.134.3.1141-1156.1978 |
p15A origin
- Cohen SN and Chang AC. Revised interpretation of the origin of the pSC101 plasmid. J Bacteriol. 1977 Nov;132(2):734-7. DOI:10.1128/jb.132.2.734-737.1977 |
pSC101 origin
- Bazaral M and Helinski DR. Circular DNA forms of colicinogenic factors E1, E2 and E3 from Escherichia coli. J Mol Biol. 1968 Sep 14;36(2):185-94. DOI:10.1016/0022-2836(68)90374-4 |
original discovery of ColE1 plasmid
- Hershfield V, Boyer HW, Yanofsky C, Lovett MA, and Helinski DR. Plasmid ColEl as a molecular vehicle for cloning and amplification of DNA. Proc Natl Acad Sci U S A. 1974 Sep;71(9):3455-9. DOI:10.1073/pnas.71.9.3455 |
use of ColEI as a vector
- ISBN:0-87969-577-3
- Anderson ES. The ecology of transferable drug resistance in the enterobacteria. Annu Rev Microbiol. 1968;22:131-80. DOI:10.1146/annurev.mi.22.100168.001023 |
Discusses transferable drug resistance in Escherichia coli (i.e. plasmids with resistance markers)
- Cohen SN and Chang AC. Recircularization and autonomous replication of a sheared R-factor DNA segment in Escherichia coli transformants. Proc Natl Acad Sci U S A. 1973 May;70(5):1293-7. DOI:10.1073/pnas.70.5.1293 |
Discusses generation of plasmids by sharing DNA, their transformation and replication.
- Cohen SN, Chang AC, Boyer HW, and Helling RB. Construction of biologically functional bacterial plasmids in vitro. Proc Natl Acad Sci U S A. 1973 Nov;70(11):3240-4. DOI:10.1073/pnas.70.11.3240 |
Discusses use of vector to propagate other DNA fragments
External links
- Novagen pET vector table
- table with links to properties/sequences of pET vectors
- note: slow to load