Escherichia coli/Knockouts: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
No edit summary |
||
Line 15: | Line 15: | ||
==Source== | ==Source== | ||
*[http://lists.syntheticbiology.org/pipermail/discuss/2006q2/000132.html Email correspondence] between John Cumbers and Wilson Wong. | *[http://lists.syntheticbiology.org/pipermail/discuss/2006q2/000132.html Email correspondence] between John Cumbers and Wilson Wong. | ||
[[Category:Escherichia coli]] |
Revision as of 15:58, 5 December 2006
The Keio collection is a collection of systematic single-gene knock-out mutants of E. coli K-12 done by the Functional Genomic Analysis of E. coli in Japan project [1].
You can request strains from them.
Request form - note that they are quoting a shipping delay of 2 months due to so many requests.
Alternatively, you can obtain the relevant plasmids for doing the knockouts yourself from the CGSC (pKD13 and pKD46 are needed most likely)[2].
References
- Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, and Mori H. Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol. 2006;2:2006.0008. DOI:10.1038/msb4100050 |
- Datsenko KA and Wanner BL. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6640-5. DOI:10.1073/pnas.120163297 |
Source
- Email correspondence between John Cumbers and Wilson Wong.