Enzyme selection for BioBricks digest

A BioBricks part:

• -----E--X---Part---S--P-----

Vector with upstream part - S,P

Downstream Insert - X,P

Upstream Insert - E,S

Vector with downstream part- E,X

E= EcoRI X= XbaI S= SpeI P= PstI

Notes

Very small (<100kb) inserts have shown to be difficult to remove from a gel purification. It is recommended that during assembly very small pieces be kept on the vector and larger pieces be used as inserts if possible.