Endy:dNTP stock solution: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
Each individual dNTP comes as 25 μmol in 100 mM solution. The lids are color-coded to each nucleic acid. | Each individual dNTP comes as 25 μmol in 100 mM solution. The lids are color-coded to each nucleic acid. | ||
My dNTP stocks are: | My dNTP stocks are: | ||
*100x: 25mM each dNTP. Combine equal volumes of 100 mM individual dNTPs. | *100x: 25mM each dNTP. Combine equal volumes of 100 mM individual dNTPs. | ||
| Line 13: | Line 6: | ||
*Working concentration: 250 μM<br> | *Working concentration: 250 μM<br> | ||
-[[User:Jkm|Jkm]] 10:04, 31 Aug 2005 (EDT) | -[[User:Jkm|Jkm]] 10:04, 31 Aug 2005 (EDT) | ||
This seems to make more sense than diluting individual dNTPs, the advantages being fewer tubes, no wondering if there's the same amount of each dNTP when you combine them, and fewer freeze-thaw cycles. | |||
[[User:ClarkeS|Sean]] 11:58, 1 Sep 2005 (EDT) | |||
Latest revision as of 08:58, 1 September 2005
Each individual dNTP comes as 25 μmol in 100 mM solution. The lids are color-coded to each nucleic acid.
My dNTP stocks are:
- 100x: 25mM each dNTP. Combine equal volumes of 100 mM individual dNTPs.
- 10x: 2.5mM each dNTP. Dilute back 100x stock 1:10.
- Working concentration: 250 μM
-Jkm 10:04, 31 Aug 2005 (EDT)
This seems to make more sense than diluting individual dNTPs, the advantages being fewer tubes, no wondering if there's the same amount of each dNTP when you combine them, and fewer freeze-thaw cycles. Sean 11:58, 1 Sep 2005 (EDT)
