Endy:Victor3 plate reader/filters: Difference between revisions
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!Use | !Use | ||
|---- | |---- | ||
|P405||Colorimetric label emission|| 405 || 10 || A1 ||Yes || | |P405||Colorimetric label emission|| 405 || 10 || A1 ||Yes || Used by Lauffenburger lab for 400nm fluorescence excitation. | ||
|- | |- | ||
|P450||Colorimetric label emission|| 450 || 6 || | |P450||Colorimetric label emission|| 450 || 6 || A6||Yes || | ||
|- | |- | ||
|P490||Colorimetric label emission|| 490 || 6 || A3||Yes || Used by Nora in Keating lab for colometric assays to measure abs. | |P490||Colorimetric label emission|| 490 || 6 || A3||Yes || Used by Nora in Keating lab for colometric assays to measure abs. | ||
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|380DF|| || 380 || 15 || --|| No?|| | |380DF|| || 380 || 15 || --|| No?|| | ||
|- | |- | ||
|P570|| || 570 || 18 || | |P570|| || 570 || 18 || Uninstalled|| || | ||
|- | |- | ||
|595DF|| || 595 || 10 || --|| || | |595DF|| || 595 || 10 || --|| || | ||
| Line 25: | Line 25: | ||
|D320||TR fluorescence excitation|| 320 || 80 || --||Yes || | |D320||TR fluorescence excitation|| 320 || 80 || --||Yes || | ||
|- | |- | ||
|F485||Fluorescein excitation|| 485 || 15 || A5||Yes ||We use this to excite GFP | |F485||Fluorescein excitation|| 485 || 15 || A5||Yes ||We use this to excite GFP. Also used by Lauffenburger lab for 485nm fluorescence excitation. | ||
|- | |- | ||
|F355|| Umbelliferone excitation|| 355 || 40 || | |F355|| Umbelliferone excitation|| 355 || 40 || Uninstalled||Yes || | ||
|- | |- | ||
|RFP-544||RFP excitation||544||?||A2||No|| | |RFP-544||RFP excitation||544||?||A2||No|| | ||
|- | |- | ||
|OD-600||Absorbance||600||?||A4||No|| | |OD-600||Absorbance||600||?||A4||No||We use this for the OD600 label | ||
|- | |- | ||
|YFP||YFP excitation||500||8||A7||No|| | |YFP||YFP excitation||500||8||A7||No|| | ||
|- | |- | ||
|CFP||CFP excitation||430||?||A8||No||This is listed as a P430, don't know if this means we shouldn't use it as a fluorescent excitation filter. | |CFP (F0439A P430)||CFP excitation||430||?||A8||No||This is listed as a P430, don't know if this means we shouldn't use it as a fluorescent excitation filter. | ||
|} | |} | ||
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!Use | !Use | ||
|---- | |---- | ||
|D615||Europium chelate emission|| 615 || 8.5 || A1||Yes || | |D615||Europium chelate emission|| 615 || 8.5 || A1||Yes || Used by Lauffenburger lab for 590nm fluorescence emission -- only okay, but better than RFP emission filter in slot B2 [F0426A 620nm]. | ||
|- | |- | ||
|D665||Europium chelate emission|| 665 || 7.5 || A8||Yes || | |D665||Europium chelate emission|| 665 || 7.5 || A8||Yes || | ||
| Line 68: | Line 68: | ||
|510DF40EM|| ? ||510 ||40?||Uninstalled|||| | |510DF40EM|| ? ||510 ||40?||Uninstalled|||| | ||
|- | |- | ||
|510DF20EM|| GFP emission ||510 ||20?||B1|||| | |510DF20EM|| GFP emission ||510 ||20?||B1|||| Used by Lauffenburger lab for both 505nm and 520nm fluorescence emission -- only okay. | ||
|- | |- | ||
|F0426A|| ||620||?||B2||No|| | |F0426A|| ||620||?||B2||No||We use this for RFP and mCherry emission. Anecdotal evidence suggests that it isn't as bright as the standard GFP label we use. | ||
|- | |- | ||
|F0436A||YFP emission ||535||?||B3||No||We also use this for GFP emission because we were picking up excitation light when using the 510 filter. | |F0436A||YFP emission ||535||?||B3||No||We also use this for GFP emission because we were picking up excitation light when using the 510 filter. | ||
Latest revision as of 09:55, 14 March 2007
Excitation Filters
| Name | Use | Transmission Peak | Bandwidth | Filter Wheel | Factory Installed | Use |
|---|---|---|---|---|---|---|
| P405 | Colorimetric label emission | 405 | 10 | A1 | Yes | Used by Lauffenburger lab for 400nm fluorescence excitation. |
| P450 | Colorimetric label emission | 450 | 6 | A6 | Yes | |
| P490 | Colorimetric label emission | 490 | 6 | A3 | Yes | Used by Nora in Keating lab for colometric assays to measure abs. |
| 380DF | 380 | 15 | -- | No? | ||
| P570 | 570 | 18 | Uninstalled | |||
| 595DF | 595 | 10 | -- | |||
| D340 | TR fluorescence excitation | 340 | 50 | Uninstalled | Yes | |
| D320 | TR fluorescence excitation | 320 | 80 | -- | Yes | |
| F485 | Fluorescein excitation | 485 | 15 | A5 | Yes | We use this to excite GFP. Also used by Lauffenburger lab for 485nm fluorescence excitation. |
| F355 | Umbelliferone excitation | 355 | 40 | Uninstalled | Yes | |
| RFP-544 | RFP excitation | 544 | ? | A2 | No | |
| OD-600 | Absorbance | 600 | ? | A4 | No | We use this for the OD600 label |
| YFP | YFP excitation | 500 | 8 | A7 | No | |
| CFP (F0439A P430) | CFP excitation | 430 | ? | A8 | No | This is listed as a P430, don't know if this means we shouldn't use it as a fluorescent excitation filter. |
Emission Filters
| Name | Use | Transmission Peak | Bandwidth | Filter Slide | Factory Installed | Use |
|---|---|---|---|---|---|---|
| D615 | Europium chelate emission | 615 | 8.5 | A1 | Yes | Used by Lauffenburger lab for 590nm fluorescence emission -- only okay, but better than RFP emission filter in slot B2 [F0426A 620nm]. |
| D665 | Europium chelate emission | 665 | 7.5 | A8 | Yes | |
| D642 | Samarium chelate emission | 642 | 6.5 | A2 | Yes | |
| D545 | Terbium chelate emission | 545 | 7.5 | A3 | Yes | |
| D572 | Dysprosium chelate emission | 572 | 7.5 | Uninstalled | Yes | |
| F535 | Fluorescein emission | 535 | 25 | A5 | Yes | |
| F460 | Umbelliferone emission | 460 | 40 | -- | Yes | |
| 620DF10EM | ? | ? | ? | Uninstalled | ||
| 630DF10 | ? | ? | ? | Uninstalled | ||
| 510DF40EM | ? | 510 | 40? | Uninstalled | ||
| 510DF20EM | GFP emission | 510 | 20? | B1 | Used by Lauffenburger lab for both 505nm and 520nm fluorescence emission -- only okay. | |
| F0426A | 620 | ? | B2 | No | We use this for RFP and mCherry emission. Anecdotal evidence suggests that it isn't as bright as the standard GFP label we use. | |
| F0436A | YFP emission | 535 | ? | B3 | No | We also use this for GFP emission because we were picking up excitation light when using the 510 filter. |
| 4D318 Corion | 460 | ? | B4 | No | ||
| F0432A D572 | 572 | ? | B8 | No |
Abbreviations:
D = Time resolved fluorometry, including LANCE
F = Fluorometry
P = Photometry
L = Luminometry
These are the recommended applications for the filters. It is not clear whether there is actually any difference between, for example, a fluorometry excitation filter and a photometry filter.
