The Endy lab has a Perkin Elmer Victor3 plate reader with 2 dispenser pumps. If you are interested in trying our plate reader or finding out more about it, contact Barry. The machine can make the following classes of measurement -
- Time-resolved fluorometry
- Fluorescence polarization
We have a variety of filters to be used with these measurements, they are described here. If you haven't used the machine before, have a look at this overview of using the plate reader. If you have used this machine or similar machines, have a look at some of the usage notes and data analysis pages listed below. All contributions are welcome.
Plate reader sign-up
We're testing a google calendar as a replacement for the old paper sign-up sheet. The calendar name is Endy Plate Reader Sign Up. You can access the sign-up sheet in a couple of ways -
- If you are a google calendar user, search public calendars for "Endy Plate Reader Sign Up" and subscribe to it. If you send me your username, I will give you edit access.
- If you don't have a google calendar account, you can sign up using your MIT email address here
- If you use a different calendar application or RSS feed reader, you can subscribe to the ical file or the xml feed that are linked below although that won't let you edit the calendar.
- The calendar should be open on the desktop of the plate reader computer.
Creating a basic protocol
Creating and choosing labels
What is a label?
Detection limits and linear range
Setting an appropriate lamp energy
Running a protocol
Counteracting evaporation in long time courses
Basic data analysis
Converting from absorbance to OD600
Data analysis tools
When things go bad
- Instrument serial number - 4206012
- Perkin Elmer Contact - 8007624000 (add a 91 prefix from an MIT phone). Choose option 3 and option 3 again to talk to tech. support.
- Endy:Victor3 Service History