Endy:Victor3 plate reader: Difference between revisions
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== | ==Introduction== | ||
The Endy lab has a [http://las.perkinelmer.com/Catalog/default.htm?CategoryID=VICTOR%3Csup%3E3%3C%2Fsup%3E Perkin Elmer Victor3] plate reader with 2 dispenser pumps. If you are interested in trying our plate reader or finding out more about it, contact [[Barry Canton|Barry]]. The machine can make the following classes of measurement - | |||
*Time-resolved fluorometry | *Time-resolved fluorometry | ||
*Fluorometry | *Fluorometry | ||
| Line 14: | Line 9: | ||
*Fluorescence polarization | *Fluorescence polarization | ||
*LANCE | *LANCE | ||
We have a variety of filters to be used with these measurements, they are described [[Endy:Victor3 plate reader/filters|here]]. If you haven't used the machine before, have a look at this [[Endy:Victor3 Getting Started|overview of using the plate reader]]. If you have used this machine or similar machines, have a look at some of the usage notes and data analysis pages listed below. All contributions are welcome. | |||
[[Endy:Victor3 | |||
[[Endy:Victor3 plate reader | |||
==Protocols== | ==Protocols== | ||
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[[Endy:Victor3 plate reader/protocol editing | Protocol editing]] | [[Endy:Victor3 plate reader/protocol editing | Protocol editing]] | ||
=== | ===Creating and choosing labels=== | ||
[[Endy:Victor3 plate reader/labels|What is a label?]]<br> | [[Endy:Victor3 plate reader/labels|What is a label?]]<br> | ||
[[Endy:Victor3 detection limits and linear range|Detection limits and linear range]]<br> | [[Endy:Victor3 detection limits and linear range|Detection limits and linear range]]<br> | ||
Revision as of 16:07, 6 January 2006
Introduction
The Endy lab has a Perkin Elmer Victor3 plate reader with 2 dispenser pumps. If you are interested in trying our plate reader or finding out more about it, contact Barry. The machine can make the following classes of measurement -
- Time-resolved fluorometry
- Fluorometry
- Photometry
- Luminometry
- Fluorescence polarization
- LANCE
We have a variety of filters to be used with these measurements, they are described here. If you haven't used the machine before, have a look at this overview of using the plate reader. If you have used this machine or similar machines, have a look at some of the usage notes and data analysis pages listed below. All contributions are welcome.
Protocols
Creating a basic protocol
Creating and choosing labels
What is a label?
Detection limits and linear range
Setting an appropriate lamp energy
Running a protocol
Counteracting evaporation in long time courses
Data Analysis
Basic data analysis
Measuring PoPS
Background measurements
Excel macro to hasten data processing
Variation in repeated measurements
Plate variation
Anecdotal
Converting from absorbance to OD600
When things go bad
- Instrument serial number - 4206012
- Perkin Elmer Contact - 8007624000 (add a 91 prefix from an MIT phone). Choose option 3 and option 3 again to talk to tech. support.
- Endy:Victor3 Service History
