Endy:Victor3 plate reader: Difference between revisions
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==Getting Started== | ==Getting Started== | ||
We have a [http://las.perkinelmer.com/Catalog/default.htm?CategoryID=VICTOR%3Csup%3E3%3C%2Fsup%3E Perkin Elmer Victor3] plate reader with 2 dispenser pumps. The plate reader can make the following classes of measurement - | |||
*Time-resolved fluorometry | |||
*Fluorometry | |||
*Photometry | |||
*Luminometry | |||
*Fluorescence polarization | |||
*LANCE | |||
[[Endy:Victor3 Getting Started|Overview of using the plate reader]]<br> | [[Endy:Victor3 Getting Started|Overview of using the plate reader]]<br> | ||
[[Endy:Victor3 plate reader/filters|Available filters]]<br> | [[Endy:Victor3 plate reader/filters|Available filters]]<br> |
Revision as of 15:41, 6 January 2006
The Perkin Elmer Victor3 Plate Reader is located in 68-558d. The manufacturer website is located here
Contact
Barry Canton is the point man in the Endy Lab if you would like to use this machine.
Getting Started
We have a Perkin Elmer Victor3 plate reader with 2 dispenser pumps. The plate reader can make the following classes of measurement -
- Time-resolved fluorometry
- Fluorometry
- Photometry
- Luminometry
- Fluorescence polarization
- LANCE
Overview of using the plate reader
Available filters
Protocols
Creating a basic protocol
Choosing labels
What is a label?
Detection limits and linear range
Setting an appropriate lamp energy
Running a protocol
Counteracting evaporation in long time courses
Data Analysis
Basic data analysis
Measuring PoPS
Background measurements
Excel macro to hasten data processing
Variation in repeated measurements
Plate variation
Anecdotal
Converting from absorbance to OD600
When things go bad
- Instrument serial number - 4206012
- Perkin Elmer Contact - 8007624000 (add a 91 prefix from an MIT phone). Choose option 3 and option 3 again to talk to tech. support.
- Endy:Victor3 Service History