Endy:Victor3 plate reader

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Plate reader sign-up)
(Plate reader sign-up)
Line 15: Line 15:
==Plate reader sign-up==
==Plate reader sign-up==
-
We're testing a google calendar as a replacement for the old paper sign-up sheet.  You can view the sign-up sheet in a couple of ways -
+
We're testing a google calendar as a replacement for the old paper sign-up sheet.  The calendar name is '''Endy Plate Reader Sign Up'''.  You can access the sign-up sheet in a couple of ways -
-
#If you an application that allows you to subscribe to iCal files, then use this [http://www.google.com/calendar/ical/endy_plate_reader@mit.edu/public/basic iCal link].
+
#If you are a google calendar user, search public calendars for "Endy Plate Reader Sign Up" and subscribe to it. If you send me your username, I will give you edit access.
-
#If you have an rss reader, you can use this [http://www.google.com/calendar/feeds/endy_plate_reader@mit.edu/public/basic xml link]. 
+
#If you don't have a google calendar account, you can sign up using your MIT email address [https://www.google.com/accounts/NewAccount?continue=http%3A%2F%2Fwww.google.com%2Fcalendar&service=cl here]  
-
#If you have a gmail account, search for "Endy Plate Reader Sign Up" in google calendar.
+
#If you use a different calendar application or RSS feed reader, you can subscribe to the ical file or the xml feed that are linked below although that won't let you edit the calendar.
-
 
+
#*[http://www.google.com/calendar/ical/endy_plate_reader@mit.edu/public/basic iCal link]
-
If you would like to sign up for time on the plate reader, you can
+
#*[http://www.google.com/calendar/feeds/endy_plate_reader@mit.edu/public/basic xml link]
-
#Send your gmail address to [[Barry Canton|me]] and I'll give you edit access to the calendar.
+
#The calendar should be open on the desktop of the plate reader computer.
-
#Even if you don't have a gmail address, you can create a google calendar account with your MIT email address.  Just go to this [https://www.google.com/accounts/NewAccount?continue=http%3A%2F%2Fwww.google.com%2Fcalendar&service=cl link] and create an account.  If you send [[Barry Canton|me]] your google account name, I'll give you edit access.
+
-
#If you don't have a gmail account, you can still view the calendar using one of the methods described above and email [[Barry Canton|Barry]] with the times for which you would like to sign up.
+
==Protocols==
==Protocols==

Revision as of 21:27, 14 May 2006

Home        Lab Members        Research        Notebooks        Publications        Internal        Contact       


Contents

Introduction

The Endy lab has a Perkin Elmer Victor3 plate reader with 2 dispenser pumps. If you are interested in trying our plate reader or finding out more about it, contact Barry. The machine can make the following classes of measurement -

  • Time-resolved fluorometry
  • Fluorometry
  • Photometry
  • Luminometry
  • Fluorescence polarization
  • LANCE

We have a variety of filters to be used with these measurements, they are described here. If you haven't used the machine before, have a look at this overview of using the plate reader. If you have used this machine or similar machines, have a look at some of the usage notes and data analysis pages listed below. All contributions are welcome.

Plate reader sign-up

We're testing a google calendar as a replacement for the old paper sign-up sheet. The calendar name is Endy Plate Reader Sign Up. You can access the sign-up sheet in a couple of ways -

  1. If you are a google calendar user, search public calendars for "Endy Plate Reader Sign Up" and subscribe to it. If you send me your username, I will give you edit access.
  2. If you don't have a google calendar account, you can sign up using your MIT email address here
  3. If you use a different calendar application or RSS feed reader, you can subscribe to the ical file or the xml feed that are linked below although that won't let you edit the calendar.
  4. The calendar should be open on the desktop of the plate reader computer.

Protocols

Creating a basic protocol

Protocol editing

Creating and choosing labels

What is a label?
Detection limits and linear range
Setting an appropriate lamp energy
Absorbance labels

Running a protocol

Arranging samples

Counteracting evaporation in long time courses

Data Analysis

Basic data analysis
Measuring PoPS
Background measurements
Measurement variation
Plate variation
Anecdotal
Converting from absorbance to OD600
Data analysis tools

When things go bad

  • Instrument serial number - 4206012
  • Perkin Elmer Contact - 8007624000 (add a 91 prefix from an MIT phone). Choose option 3 and option 3 again to talk to tech. support.
  • Endy:Victor3 Service History
Personal tools