Endy:Victor3 plate reader

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*Time-resolved fluorometry
*Time-resolved fluorometry
*Fluorometry
*Fluorometry
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*Photometry
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*[[Endy:Victor3 plate reader/Absorbance labels|Photometry]]
*Luminometry
*Luminometry
*Fluorescence polarization
*Fluorescence polarization
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==Plate reader schedule==
==Plate reader schedule==
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You can view the schedule for the plate reader on the google calendar below.  You need to be subscribed to the calendar in order to reserve time on the plate reader.  You can subscribe to the calendar in a number of ways.
 
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#If you are a google calendar user, subscribe using the link below the calendar.  If you send me your username, I will give you edit access.
 
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#If you don't have a google calendar account, you can sign up using your MIT email address [https://www.google.com/accounts/NewAccount?continue=http%3A%2F%2Fwww.google.com%2Fcalendar&service=cl here]
 
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#If you use a different calendar application or RSS feed reader, you can subscribe to the ical file or the xml feed that are linked below although that won't let you edit the calendar.
 
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#*[http://www.google.com/calendar/ical/endy_plate_reader@mit.edu/public/basic iCal link]
 
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#*[http://www.google.com/calendar/feeds/endy_plate_reader@mit.edu/public/basic xml link]
 
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#The calendar should be open on the desktop of the plate reader computer.
 
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<html>
<html>
<center>
<center>
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<iframe src="http://www.google.com/calendar/embed?src=endy_plate_reader%40mit.edu&title=Plate%20Reader%20Signup&chrome=NAVIGATION&height=588" style=" border-width:0 " width="640" frameborder="0" height="700"></iframe>
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<iframe src="http://www.google.com/calendar/embed?src=endy_plate_reader%40mit.edu&title=Plate%20Reader%20Signup&chrome=NAVIGATION&height=588" style=" border-width:0 " width="640" frameborder="0" height="630"></iframe>
</center>
</center>
</html>
</html>
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[[Endy:Victor3 plate reader/Schedule Access|How to sign up]]
==Protocols==
==Protocols==
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===Creating a basic protocol===
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===Programming a basic protocol===
[[Endy:Victor3 plate reader/protocol editing | Protocol editing]]
[[Endy:Victor3 plate reader/protocol editing | Protocol editing]]
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[[Endy:Victor3 lamp energy|Setting an appropriate lamp energy]]<br>
[[Endy:Victor3 lamp energy|Setting an appropriate lamp energy]]<br>
[[Endy:Victor3 plate reader/Absorbance labels|Absorbance labels]]<br>
[[Endy:Victor3 plate reader/Absorbance labels|Absorbance labels]]<br>
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[[Endy:Victor3 plate reader/Changing filter slides|Changing filter slides]]<br>
===Running a protocol===
===Running a protocol===

Current revision

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Contents

Introduction

The Endy lab has a Perkin Elmer Victor3 plate reader with 2 dispenser pumps. If you are interested in trying our plate reader or finding out more about it, contact Barry. The machine can make the following classes of measurement -

  • Time-resolved fluorometry
  • Fluorometry
  • Photometry
  • Luminometry
  • Fluorescence polarization
  • LANCE

We have a variety of filters to be used with these measurements, they are described here. If you haven't used the machine before, have a look at this overview of using the plate reader. If you have used this machine or similar machines, have a look at some of the usage notes and data analysis pages listed below. All contributions are welcome.

Plate reader schedule

How to sign up

Protocols

Programming a basic protocol

Protocol editing

Creating and choosing labels

What is a label?
Detection limits and linear range
Setting an appropriate lamp energy
Absorbance labels
Changing filter slides

Running a protocol

Arranging samples

Counteracting evaporation in long time courses

Data Analysis

Basic data analysis
Calculating fluorescent protein synthesis
Measuring PoPS
Background measurements
Measurement variation
Plate variation
Anecdotal
Converting from absorbance to OD600
Data analysis tools

When things go bad

  • Instrument serial number - 4206012
  • Perkin Elmer Contact - 8007624000 (add a 91 prefix from an MIT phone). Choose option 3 and option 3 again to talk to tech. support.
  • Endy:Victor3 Service History
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