Endy:Victor3 plate reader

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{{EndyLab}}
{{EndyLab}}
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The Perkin Elmer Victor<sup>3</sup> Plate Reader is located in 68-558d. The manufacturer website is located [http://las.perkinelmer.com/Catalog/default.htm?CategoryID=VICTOR%3Csup%3E3%3C%2Fsup%3E here]
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== Usage Notes ==
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==Introduction==
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The Endy lab has a [http://las.perkinelmer.com/Catalog/default.htm?CategoryID=VICTOR%3Csup%3E3%3C%2Fsup%3E Perkin Elmer Victor3] plate reader with 2 dispenser pumps.  If you are interested in trying our plate reader or finding out more about it, contact [[Barry Canton|Barry]].  The machine can make the following classes of measurement -
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*Time-resolved fluorometry
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*Fluorometry
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*[[Endy:Victor3 plate reader/Absorbance labels|Photometry]]
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*Luminometry
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*Fluorescence polarization
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*LANCE
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We have a variety of filters to be used with these measurements, they are described [[Endy:Victor3 plate reader/filters|here]].  If you haven't used the machine before, have a look at this [[Endy:Victor3 Getting Started|overview of using the plate reader]].  If you have used this machine or similar machines, have a look at some of the usage notes and data analysis pages listed below.  All contributions are welcome.
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[[Endy:Victor3 detection limits and linear range|Detection limits and linear range]]
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==Plate reader schedule==
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<html>
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<center>
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<iframe src="http://www.google.com/calendar/embed?src=endy_plate_reader%40mit.edu&title=Plate%20Reader%20Signup&chrome=NAVIGATION&height=588" style=" border-width:0 " width="640" frameborder="0" height="630"></iframe>
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</center>
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</html>
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[[Endy:Victor3 plate reader/Schedule Access|How to sign up]]
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[[Endy:Victor3 lamp energy|Lamp energy]]
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==Protocols==
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===Programming a basic protocol===
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[[Endy:Victor3 plate reader/protocol editing | Protocol editing]]
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[[Endy:Victor3 absorbance labels|Absorbance labels]]
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===Creating and choosing labels===
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[[Endy:Victor3 plate reader/labels|What is a label?]]<br>
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[[Endy:Victor3 plate reader/detection limits and linear range|Detection limits and linear range]]<br>
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[[Endy:Victor3 lamp energy|Setting an appropriate lamp energy]]<br>
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[[Endy:Victor3 plate reader/Absorbance labels|Absorbance labels]]<br>
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[[Endy:Victor3 plate reader/Changing filter slides|Changing filter slides]]<br>
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===Running a protocol===
[[Endy:Victor3 arranging samples|Arranging samples]]
[[Endy:Victor3 arranging samples|Arranging samples]]
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[[Endy:Victor3 Excel macro to hasten data processing|Excel macro to hasten data processing]]
 
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[[Endy:Victor3 variation in repeated measurements|Variation in repeated measurements]]
 
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[[Endy:Victor3 plate variation| Plate variation]]
 
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== Protocols ==
 
[[Endy:Victor3 counteracting evaporation in long time courses|Counteracting evaporation in long time courses]]
[[Endy:Victor3 counteracting evaporation in long time courses|Counteracting evaporation in long time courses]]
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== Contact ==
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==Data Analysis==
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[[Endy:Basic data analysis on a plate reader|Basic data analysis]]<br>
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[[Endy:Victor3 Calculating fluorescent protein synthesis|Calculating fluorescent protein synthesis]]<br>
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[[Endy:Measuring PoPS on a plate reader|Measuring PoPS]]<br>
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[[Endy:Victor3 Absorbance and fluorescence backgrounds|Background measurements]]<br>
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[[Endy:Victor3 plate reader/Measurement variation|Measurement variation]]<br>
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[[Endy:Victor3 plate variation| Plate variation]]<br>
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[[Endy:Victor3 Anecdotal| Anecdotal]]<br>
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[[Endy:Victor3 absorbance labels|Converting from absorbance to OD600]]<br>
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[[Endy:Victor3 plate reader/Data analysis tools|Data analysis tools]]<br>
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[[Barry Canton]] is the point man in the [[Endy Lab]] if you would like to use this machine.
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==When things go bad==
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*'''Instrument serial number''' - 4206012
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*'''Perkin Elmer Contact''' - 8007624000 (add a 91 prefix from an MIT phone).  Choose option 3 and option 3 again to talk to tech. support.
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*[[Endy:Victor3 Service History]]
[[Category: Equipment]]
[[Category: Equipment]]
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Current revision

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Contents

Introduction

The Endy lab has a Perkin Elmer Victor3 plate reader with 2 dispenser pumps. If you are interested in trying our plate reader or finding out more about it, contact Barry. The machine can make the following classes of measurement -

  • Time-resolved fluorometry
  • Fluorometry
  • Photometry
  • Luminometry
  • Fluorescence polarization
  • LANCE

We have a variety of filters to be used with these measurements, they are described here. If you haven't used the machine before, have a look at this overview of using the plate reader. If you have used this machine or similar machines, have a look at some of the usage notes and data analysis pages listed below. All contributions are welcome.

Plate reader schedule

How to sign up

Protocols

Programming a basic protocol

Protocol editing

Creating and choosing labels

What is a label?
Detection limits and linear range
Setting an appropriate lamp energy
Absorbance labels
Changing filter slides

Running a protocol

Arranging samples

Counteracting evaporation in long time courses

Data Analysis

Basic data analysis
Calculating fluorescent protein synthesis
Measuring PoPS
Background measurements
Measurement variation
Plate variation
Anecdotal
Converting from absorbance to OD600
Data analysis tools

When things go bad

  • Instrument serial number - 4206012
  • Perkin Elmer Contact - 8007624000 (add a 91 prefix from an MIT phone). Choose option 3 and option 3 again to talk to tech. support.
  • Endy:Victor3 Service History
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