Endy:Victor3 plate reader

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Plate reader sign-up sheet)
Current revision (13:38, 29 October 2007) (view source)
(Introduction)
 
(10 intermediate revisions not shown.)
Line 8: Line 8:
*Time-resolved fluorometry
*Time-resolved fluorometry
*Fluorometry
*Fluorometry
-
*Photometry
+
*[[Endy:Victor3 plate reader/Absorbance labels|Photometry]]
*Luminometry
*Luminometry
*Fluorescence polarization
*Fluorescence polarization
Line 14: Line 14:
We have a variety of filters to be used with these measurements, they are described [[Endy:Victor3 plate reader/filters|here]].  If you haven't used the machine before, have a look at this [[Endy:Victor3 Getting Started|overview of using the plate reader]].  If you have used this machine or similar machines, have a look at some of the usage notes and data analysis pages listed below.  All contributions are welcome.
We have a variety of filters to be used with these measurements, they are described [[Endy:Victor3 plate reader/filters|here]].  If you haven't used the machine before, have a look at this [[Endy:Victor3 Getting Started|overview of using the plate reader]].  If you have used this machine or similar machines, have a look at some of the usage notes and data analysis pages listed below.  All contributions are welcome.
-
==Plate reader sign-up==
+
==Plate reader schedule==
-
We're testing a google calendar as a replacement for the old paper sign-up sheet.  You can view the sign-up sheet in a couple of ways -
+
<html>
-
#If you an application that allows you to subscribe to iCal files, then use this [http://www.google.com/calendar/ical/do3vfe9fl5osapua1d6avdnl5k@group.calendar.google.com/public/basic iCal link].
+
<center>
-
#If you have an rss reader, you can use this [http://www.google.com/calendar/feeds/do3vfe9fl5osapua1d6avdnl5k@group.calendar.google.com/public/basic xml link]. 
+
<iframe src="http://www.google.com/calendar/embed?src=endy_plate_reader%40mit.edu&title=Plate%20Reader%20Signup&chrome=NAVIGATION&height=588" style=" border-width:0 " width="640" frameborder="0" height="630"></iframe>
-
#If you have a gmail account, search for "Endy Lab Plate Reader" in google calendar.
+
</center>
-
 
+
</html>
-
If you would like to sign up for time on the plate reader, you can
+
[[Endy:Victor3 plate reader/Schedule Access|How to sign up]]
-
#Send your gmail address to [[Barry Canton|me]] and I'll give you edit access to the calendar.
+
-
#Even if you don't have a gmail address, you can create a google calendar account with your MIT email address.  Just go to this [https://www.google.com/accounts/NewAccount?continue=http%3A%2F%2Fwww.google.com%2Fcalendar&service=cl link] and create an account.
+
-
#If you don't have a gmail account, you can still view the calendar using one of the methods described above and email [[Barry Canton|Barry]] with the times for which you would like to sign up.
+
==Protocols==
==Protocols==
-
===Creating a basic protocol===
+
===Programming a basic protocol===
[[Endy:Victor3 plate reader/protocol editing | Protocol editing]]
[[Endy:Victor3 plate reader/protocol editing | Protocol editing]]
Line 34: Line 31:
[[Endy:Victor3 lamp energy|Setting an appropriate lamp energy]]<br>
[[Endy:Victor3 lamp energy|Setting an appropriate lamp energy]]<br>
[[Endy:Victor3 plate reader/Absorbance labels|Absorbance labels]]<br>
[[Endy:Victor3 plate reader/Absorbance labels|Absorbance labels]]<br>
 +
[[Endy:Victor3 plate reader/Changing filter slides|Changing filter slides]]<br>
===Running a protocol===
===Running a protocol===
Line 42: Line 40:
==Data Analysis==
==Data Analysis==
[[Endy:Basic data analysis on a plate reader|Basic data analysis]]<br>
[[Endy:Basic data analysis on a plate reader|Basic data analysis]]<br>
 +
[[Endy:Victor3 Calculating fluorescent protein synthesis|Calculating fluorescent protein synthesis]]<br>
[[Endy:Measuring PoPS on a plate reader|Measuring PoPS]]<br>
[[Endy:Measuring PoPS on a plate reader|Measuring PoPS]]<br>
[[Endy:Victor3 Absorbance and fluorescence backgrounds|Background measurements]]<br>
[[Endy:Victor3 Absorbance and fluorescence backgrounds|Background measurements]]<br>

Current revision

Home        Lab Members        Research        Notebooks        Publications        Internal        Contact       


Contents

Introduction

The Endy lab has a Perkin Elmer Victor3 plate reader with 2 dispenser pumps. If you are interested in trying our plate reader or finding out more about it, contact Barry. The machine can make the following classes of measurement -

  • Time-resolved fluorometry
  • Fluorometry
  • Photometry
  • Luminometry
  • Fluorescence polarization
  • LANCE

We have a variety of filters to be used with these measurements, they are described here. If you haven't used the machine before, have a look at this overview of using the plate reader. If you have used this machine or similar machines, have a look at some of the usage notes and data analysis pages listed below. All contributions are welcome.

Plate reader schedule

How to sign up

Protocols

Programming a basic protocol

Protocol editing

Creating and choosing labels

What is a label?
Detection limits and linear range
Setting an appropriate lamp energy
Absorbance labels
Changing filter slides

Running a protocol

Arranging samples

Counteracting evaporation in long time courses

Data Analysis

Basic data analysis
Calculating fluorescent protein synthesis
Measuring PoPS
Background measurements
Measurement variation
Plate variation
Anecdotal
Converting from absorbance to OD600
Data analysis tools

When things go bad

  • Instrument serial number - 4206012
  • Perkin Elmer Contact - 8007624000 (add a 91 prefix from an MIT phone). Choose option 3 and option 3 again to talk to tech. support.
  • Endy:Victor3 Service History
Personal tools