Endy:Victor3 Absorbance and fluorescence backgrounds
Sample | Absorbance | GFP-Separated |
---|---|---|
Empty well | 0.032 | 941 |
Water | 0.042 | 924 |
EZ media + 0.4% Glycerol | 0.038 | 1035 |
EZ media + 0.4% Glycerol + Amp | 0.04 | 1003 |
EZ media + 0.4% Glycerol + Tet | 0.047 | 943 |
EZ media + 0.4% Glycerol + Amp + Tet | 0.048 | 978 |
EZ media + 0.4% Glucose + Amp + Tet | 0.047 | 1708 |
EZ media + 0.4% Glycerol + Kan | 0.046 | 833 |
EZ media + 0.4% Glycerol + Strep | 0.055 | 738 |
This plot shows the background fluorescence of EZ rich media from Teknova + 0.4% glycerol + antibiotic over time. This is data from two separate runs done on different days (circles and squares). Each run is an average of 3 replicates.
This experiment was done on the Endy lab Victor3 plate reader using 96 well plates from the Endy lab but using media and antibiotic from the Knight Lab.
The cause of the seemingly random spikes in fluorescence for the EZ rich media + 0.4% glycerol supplemented with kanamycin are unknown.
This plot is intended to demonstrate fluctuation in background (media-only) fluorescence measurements over time. This data is very preliminary and has not been independently verified. Use at your own risk!
This plot shows the background absorbance of EZ rich media from Teknova + 0.4% glycerol + antibiotic over time. This is data from two separate runs done on different days (circles and squares). Each run is an average of 3 replicates.
This experiment was done on the Endy lab Victor3 plate reader using 96 well plates from the Endy lab but using media and antibiotic from the Knight Lab.
The cause of the slight increase over time in absorbance of the EZ rich media + 0.4% glycerol supplemented with kanamycin is unknown. This may be due to human error (possibly well contamination?).
This plot is intended to demonstrate fluctuation in background (media-only) absorbance measurements over time. This data is very preliminary and has not been independently verified. Use at your own risk!