Endy:Victor3 Absorbance and fluorescence backgrounds: Difference between revisions
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[[Image:Victor3platereadermediabackground.jpg|thumb|700px|left|'''Plot of media fluorescence (measured using GFP filters) versus time''']] | |||
This plot shows the background fluorescence of [[Neidhardt EZ Rich Defined | EZ rich media]] from Teknova + 0.4% glycerol + antibiotic over time. This is data from two separate runs done on different days (circles and squares). | |||
This experiment was done on the Endy lab Victor3 plate reader using 96 well plates from the Endy lab but using media and antibiotic from the [[Knight Lab]]. | |||
The cause of the random spikes in fluorescence for the EZ rich media + 0.4% glycerol supplemented with kanamycin are unknown. | |||
Revision as of 13:16, 2 March 2006
| Sample | Absorbance | GFP-Separated |
|---|---|---|
| Empty well | 0.032 | 941 |
| Water | 0.042 | 924 |
| EZ media + 0.4% Glycerol | 0.038 | 1035 |
| EZ media + 0.4% Glycerol + Amp | 0.04 | 1003 |
| EZ media + 0.4% Glycerol + Tet | 0.047 | 943 |
| EZ media + 0.4% Glycerol + Amp + Tet | 0.048 | 978 |
| EZ media + 0.4% Glucose + Amp + Tet | 0.047 | 1708 |
This plot shows the background fluorescence of EZ rich media from Teknova + 0.4% glycerol + antibiotic over time. This is data from two separate runs done on different days (circles and squares).
This experiment was done on the Endy lab Victor3 plate reader using 96 well plates from the Endy lab but using media and antibiotic from the Knight Lab.
The cause of the random spikes in fluorescence for the EZ rich media + 0.4% glycerol supplemented with kanamycin are unknown.
