Endy:STN Chemostat Design

Contact Caitlin Conboy, Jennifer Braff.

This is a flexible design for building a chemostat from off-the-shelf parts. The number of chemostat chambers and the culture volume per chamber are adjustable.

Also see Endy:Chemostat for an alternative chemostat chamber design and additional info on running chemostats.

Design Images

Simple schematic for a single chemostat chamber.

Photo of a six-chamber chemostat. Built by cmc and jcb.

Components

This page is in progress

Equipment

• Circulating Water Bath
  • VWR
• Peristaltic pump
  • L/S peristaltic pump from Cole Parmer. Which pump, specifically?
• Bubbler (Acquarium bubbler)

Glassware

• 1L flasks. Need one per type of media to be used in the chemostat, plus one or more to collect the effluent.
• Rubber caps for 1L flasks with two drilled holes
• Glass tubing
• 25mm glass test tubes, one per chemostat chamber

Tubing, Connectors

• Silicone tubing
• Connectors, various, specify
• Three-way stopcock
  • Cole Parmer luer fittings, chrome-plated brass three-way stopcock

Other

• Needles, 2 per chamber
  • 18 gauge deflected point non-coring septum penetration needles (Popper).
• Tube Rack (to hold the chemostat chambers upright in the water bath)
  • USA Scientific
• Syringes, to innoculate and sample from chemostat

Operation

See Endy:STN Chemostat Protocol