Endy:STN Chemostat Design: Difference between revisions
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Contact [[Caitlin Conboy]], [[Jennifer Braff]] | Contact [[Caitlin Conboy]], [[Jennifer Braff]]. | ||
== | This is a flexible design for building a chemostat from off-the-shelf parts. The number of chemostat chambers and the culture volume per chamber are adjustable. | ||
[[Image:STN Chemostat Schematic.png|left| | |||
[[Image:STN Chemostat Photo.png| | Also see [[Endy:Chemostat]] for an alternative chemostat chamber design and additional info on running chemostats. | ||
==Design Images== | |||
[[Image:STN Chemostat Schematic.png|left|240px|thumb|'''Simple schematic for a single chemostat chamber.''']] | |||
[[Image:STN Chemostat Photo.png|left|160px|thumb|'''Photo of a six-chamber chemostat.''' Built by [[Caitlin Conboy|cmc]] and [[Jennifer Braff|jcb]].]] | |||
<br style="clear:both" /> | |||
==Components== | ==Components== | ||
''This page is in progress'' | |||
===Equipment=== | |||
*Circulating Water Bath | |||
**VWR | |||
*Peristaltic pump | |||
**[http://www.coleparmer.com/catalog/Product_index.asp?cls=4440|Masterflex L/S peristaltic pump] from Cole Parmer. ''Which pump, specifically?'' | |||
*Bubbler (Acquarium bubbler) | |||
===Glassware=== | |||
*1L flasks. Need one per type of media to be used in the chemostat, plus one or more to collect the effluent. | |||
*Rubber caps for 1L flasks with two drilled holes | |||
*Glass tubing | |||
*25mm glass test tubes, one per chemostat chamber | |||
===Tubing, Connectors=== | |||
*Silicone tubing | |||
*Connectors, ''various, specify'' | |||
*Three-way stopcock | |||
**Cole Parmer luer fittings, chrome-plated brass three-way stopcock | |||
===Other=== | |||
*Needles, 2 per chamber | |||
**18 gauge deflected point non-coring septum penetration needles (Popper). | |||
*Tube Rack (to hold the chemostat chambers upright in the water bath) | |||
**USA Scientific | |||
*Syringes, to innoculate and sample from chemostat | |||
==Assembly== | ==Assembly== | ||
Latest revision as of 12:36, 20 June 2006
Contact Caitlin Conboy, Jennifer Braff.
This is a flexible design for building a chemostat from off-the-shelf parts. The number of chemostat chambers and the culture volume per chamber are adjustable.
Also see Endy:Chemostat for an alternative chemostat chamber design and additional info on running chemostats.
Design Images
Components
This page is in progress
Equipment
- Circulating Water Bath
- VWR
- Peristaltic pump
- L/S peristaltic pump from Cole Parmer. Which pump, specifically?
- Bubbler (Acquarium bubbler)
Glassware
- 1L flasks. Need one per type of media to be used in the chemostat, plus one or more to collect the effluent.
- Rubber caps for 1L flasks with two drilled holes
- Glass tubing
- 25mm glass test tubes, one per chemostat chamber
Tubing, Connectors
- Silicone tubing
- Connectors, various, specify
- Three-way stopcock
- Cole Parmer luer fittings, chrome-plated brass three-way stopcock
Other
- Needles, 2 per chamber
- 18 gauge deflected point non-coring septum penetration needles (Popper).
- Tube Rack (to hold the chemostat chambers upright in the water bath)
- USA Scientific
- Syringes, to innoculate and sample from chemostat
