Endy:Preparing Antibiotic Stocks
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==[[Kanamycin]]== | ==[[Kanamycin]]== | ||
| + | ===Stocks & Usage=== | ||
| + | *'''Stock Concentration''' - 10mg/ml in H<sub>2</sub>O | ||
| + | *'''Aliquots''' - 200μl and 1ml | ||
| + | *'''Working Concentration''' - 20μg/ml | ||
| + | ===Preparation of 80ml stock solution=== | ||
| + | *Kanamycin is kept in the 4C fridge in 68-564D. It is light sensitive. | ||
| + | *Weigh 800μg of kanamycin into a small weigh boat. | ||
| + | *Add 80ml of milliQ to a 250ml bottle. | ||
| + | *Add the kanamycin to the milliQ | ||
| + | *Mix/vortex so all the kanamycin goes into solution. | ||
| + | *Filter sterilize the solution into a falcon tube using a 20ml syringe and a 200nm filter. | ||
| + | *Aliquot into pcr tubes and 1.7ml eppendorfs. | ||
| + | *Store at -20C. | ||
| + | *Store the small aliquots in the small box and the big aliquots in the larger box. | ||
==[[Tetracycline]]== | ==[[Tetracycline]]== | ||
Revision as of 21:00, 9 July 2005
Contents |
Ampicillin
Stocks & Usage
- Stock Concentration - 50mg/ml in H2O
- Aliquots - 100μl and 100μl
- Working Concentration - 50μg/ml
Preparation of 80ml stock solution
- Ampicillin is kept in the 4C fridge in 68-564D. It is light sensitive.
- Weigh 4g of ampicillin into a small weigh boat.
- Add 80ml of milliQ to a 250ml bottle.
- Add the ampicillin to the milliQ
- Mix/vortex so all the ampicillin goes into solution.
- Filter sterilize the solution into a falcon tube using a 20ml syringe and a 200nm filter.
- Aliquot into pcr tubes and 1.7ml eppendorfs.
- Store at -20C.
- Store the small aliquots in the small box and the big aliquots in the larger box.
Kanamycin
Stocks & Usage
- Stock Concentration - 10mg/ml in H2O
- Aliquots - 200μl and 1ml
- Working Concentration - 20μg/ml
Preparation of 80ml stock solution
- Kanamycin is kept in the 4C fridge in 68-564D. It is light sensitive.
- Weigh 800μg of kanamycin into a small weigh boat.
- Add 80ml of milliQ to a 250ml bottle.
- Add the kanamycin to the milliQ
- Mix/vortex so all the kanamycin goes into solution.
- Filter sterilize the solution into a falcon tube using a 20ml syringe and a 200nm filter.
- Aliquot into pcr tubes and 1.7ml eppendorfs.
- Store at -20C.
- Store the small aliquots in the small box and the big aliquots in the larger box.
Tetracycline
Stocks & Usage
- Stock Concentration - 5mg/ml in 70% Ethanol
- Aliquots - 200μl and 1ml
- Working Concentration - 20μg/ml
Preparation of 80ml stock solution
- Tetracycline is kept in the 4C fridge in 68-564D. It is light sensitive.
- Weigh 400mg of tetracyline HCL into a small weigh boat.
- Dilute 95% Ethanol to 70% using milliQ water.
- Adding 20ml of milliQ to 60ml of 95% ethanol gives 80ml of 71% ethanol.
- Add 80ml of 70% Ethanol to a 250ml bottle.
- Add the tetracycline HCL to the ethanol.
- Mix/vortex vigorously so all the tetracycline goes into solution.
- Filter sterilize the solution into a falcon tube using a 20ml syringe and a 200nm filter.
- Aliquot into pcr tubes and 1.7ml eppendorfs.
- Store at -20C and protect any unused stock solution from light.
- Store the small aliquots in the small box and the big aliquots in the larger box.
