Endy:Preparing Antibiotic Stocks: Difference between revisions
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===Preparation of 80ml stock solution=== | ===Preparation of 80ml stock solution=== | ||
*Ampicillin is kept in the 4C fridge in 68-564D. It is light sensitive. | *Ampicillin is kept in the 4C fridge in 68-564D. It is light sensitive. | ||
*Weigh | *Weigh 4g of ampicillin into a small weigh boat. | ||
*Add 80ml of milliQ to a 250ml bottle. | |||
*Add the ampicillin to the milliQ | |||
*Add 80ml of | *Mix/vortex so all the ampicillin goes into solution. | ||
*Add the | |||
*Mix/vortex | |||
*Filter sterilize the solution into a falcon tube using a 20ml syringe and a 200nm filter. | *Filter sterilize the solution into a falcon tube using a 20ml syringe and a 200nm filter. | ||
*Aliquot into pcr tubes and 1.7ml eppendorfs. | *Aliquot into pcr tubes and 1.7ml eppendorfs. | ||
*Store at -20C | *Store at -20C. | ||
*Store the small aliquots in the small box and the big aliquots in the larger box. | *Store the small aliquots in the small box and the big aliquots in the larger box. | ||
Revision as of 17:57, 9 July 2005
Ampicillin
Stocks & Usage
- Stock Concentration - 50mg/ml in H2O
- Aliquots - 100μl and 100μl
- Working Concentration - 50μg/ml
Preparation of 80ml stock solution
- Ampicillin is kept in the 4C fridge in 68-564D. It is light sensitive.
- Weigh 4g of ampicillin into a small weigh boat.
- Add 80ml of milliQ to a 250ml bottle.
- Add the ampicillin to the milliQ
- Mix/vortex so all the ampicillin goes into solution.
- Filter sterilize the solution into a falcon tube using a 20ml syringe and a 200nm filter.
- Aliquot into pcr tubes and 1.7ml eppendorfs.
- Store at -20C.
- Store the small aliquots in the small box and the big aliquots in the larger box.
Kanamycin
Tetracycline
Stocks & Usage
- Stock Concentration - 5mg/ml in 70% Ethanol
- Aliquots - 200μl and 1ml
- Working Concentration - 20μg/ml
Preparation of 80ml stock solution
- Tetracycline is kept in the 4C fridge in 68-564D. It is light sensitive.
- Weigh 400mg of tetracyline HCL into a small weigh boat.
- Dilute 95% Ethanol to 70% using milliQ water.
- Adding 20ml of milliQ to 60ml of 95% ethanol gives 80ml of 71% ethanol.
- Add 80ml of 70% Ethanol to a 250ml bottle.
- Add the tetracycline HCL to the ethanol.
- Mix/vortex vigorously so all the tetracycline goes into solution.
- Filter sterilize the solution into a falcon tube using a 20ml syringe and a 200nm filter.
- Aliquot into pcr tubes and 1.7ml eppendorfs.
- Store at -20C and protect any unused stock solution from light.
- Store the small aliquots in the small box and the big aliquots in the larger box.
