Endy:Making a long term stock of bacteria: Difference between revisions
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==Method== | ==Method== | ||
*Add | *Add 1 ml of 40% glycerol in H<sub>2</sub>O to a cryogenic vial. | ||
*Add | *Add 1 ml sample from the culture of bacteria to be stored. | ||
*Gently vortex the cryogenic vial to ensure the culture and glycerol is well mixed. | *Gently vortex the cryogenic vial to ensure the culture and glycerol is well-mixed. | ||
**Alternatively, pipet to mix. | **Alternatively, pipet to mix. | ||
*Use a tough spot to put the name of the strain or some useful identifier on the top of the vial. | *Use a tough spot to put the name of the strain or some useful identifier on the top of the vial. | ||
*On the side of the vial list all relevant information - part, vector, strain, date, researcher etc. | *On the side of the vial list all relevant information - part, vector, strain, date, researcher, etc. | ||
*Store in a freezer box in a -80C freezer. Remember to record where the vial is stored for fast retrieval later. | *Store in a freezer box in a -80C freezer. Remember to record where the vial is stored for fast retrieval later. | ||
==Notes== | ==Notes== | ||
*While it is possible to make a long term stock from cells in stationary phase, optimally your culture should be in logarithmic growth phase. | *While it is possible to make a long term stock from cells in stationary phase, optimally your culture should be in logarithmic growth phase. | ||
Revision as of 11:49, 14 September 2006
Back to Making a long term stock of bacteria
Materials
- 40% glycerol solution
- Cryogenic vials
Method
- Add 1 ml of 40% glycerol in H2O to a cryogenic vial.
- Add 1 ml sample from the culture of bacteria to be stored.
- Gently vortex the cryogenic vial to ensure the culture and glycerol is well-mixed.
- Alternatively, pipet to mix.
- Use a tough spot to put the name of the strain or some useful identifier on the top of the vial.
- On the side of the vial list all relevant information - part, vector, strain, date, researcher, etc.
- Store in a freezer box in a -80C freezer. Remember to record where the vial is stored for fast retrieval later.
Notes
- While it is possible to make a long term stock from cells in stationary phase, optimally your culture should be in logarithmic growth phase.
