Endy:Making a long term stock of bacteria: Difference between revisions
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*Add 1ml sample from the culture of bacteria to be stored. | *Add 1ml sample from the culture of bacteria to be stored. | ||
*Vortex the cryogenic vial to ensure the culture and glycerol is well mixed. | *Vortex the cryogenic vial to ensure the culture and glycerol is well mixed. | ||
**Alternatively, pipet to mix. | |||
*Use a tough spot to put the name of the strain or some useful identifier on the top of the vial. | *Use a tough spot to put the name of the strain or some useful identifier on the top of the vial. | ||
*On the side of the vial list all relevant information - part, vector, strain, date, researcher etc. | *On the side of the vial list all relevant information - part, vector, strain, date, researcher etc. | ||
Revision as of 10:28, 20 January 2006
Back to Making a long term stock of bacteria
Materials
- 40% glycerol solution
- Cryogenic vials
Method
- Add 1ml of 40% glycerol in H2O to a cryongenic vial.
- Add 1ml sample from the culture of bacteria to be stored.
- Vortex the cryogenic vial to ensure the culture and glycerol is well mixed.
- Alternatively, pipet to mix.
- Use a tough spot to put the name of the strain or some useful identifier on the top of the vial.
- On the side of the vial list all relevant information - part, vector, strain, date, researcher etc.
- Store in a freezer box in a -80C freezer. Remember to record where the vial is stored for faster retrieval later.
Notes
- While it is possible to make a long term stock from cells in stationary phase, optimally your culture should be in logarithmic growth phase.
