Endy:M9 medium/minimal

This is our protocol for making minimal M9 medium.

Component Concentrations

• 1x M9 salts
• 2mM MgSO₄
• 0.1mM CaCl₂
• 0.4% carbon source (e.g. glycerol, glucose, etc.)
• In sterile H₂O

Protocol

For 1L of media

• 500ml 2xM9 salts ∗
• 20ml 0.1M MgSO₄ ∗
• 200μl 0.5M CaCl₂ ∗
• 469.8ml sterile deionized H₂O ∗ (if carbon source to be added)
• 479.8ml sterile deionized H₂O ∗ (if no carbon source to be added, useful as a control for sugar metabolism)

Combine above solutions using sterile technique. (May notice some precipitation during preparation but precipitate should go back into solution once volume is brought up to 1l with sterile H₂O.)
Add antibiotic as appropriate and store at 4°C

∗ Can be obtained from the media room