Endy:Library-based construction: Difference between revisions
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=="Fixing" several broken inverters== | =="Fixing" several broken inverters== | ||
We will demonstrate the utility of the [[PoPS device screening plasmid|screening plasmid]] for fixing "broken" inverters via directed evolution. Preliminary results with the tetR-based inverter <bbpart>Q04400</bbpart> showed early success of this method. | We will demonstrate the utility of the [[PoPS device screening plasmid|screening plasmid]] for fixing "broken" inverters via directed evolution. Preliminary results with the tetR-based inverter <bbpart>Q04400</bbpart> showed early success of this method. | ||
==Procedure== | |||
[[Endy:Library-based construction/Inverter library protocol]] | |||
==Reshmaverters== | |||
We are working with Reshma Shetty to test her new inverter designs. | |||
*[[Knight:Evolving Reshmaverters]] - project page. | |||
===Results=== | |||
==TetR== | ==TetR== | ||
===Results=== | |||
Need to port the results described [[:Image:JK.SyntheticBiology2.0.Poster.pdf|here]]. | |||
==Mnt== | |||
==penI== | |||
===Results=== | |||
==Contact== | ==Contact== | ||
*[[Kelly Chang]] | *[[Kelly Chang]] | ||
*[[Jason Kelly]] | *[[Jason Kelly]] | ||
Latest revision as of 11:49, 17 July 2006
This is a work in progress, bear with us.
"Fixing" several broken inverters
We will demonstrate the utility of the screening plasmid for fixing "broken" inverters via directed evolution. Preliminary results with the tetR-based inverter <bbpart>Q04400</bbpart> showed early success of this method.
Procedure
Endy:Library-based construction/Inverter library protocol
Reshmaverters
We are working with Reshma Shetty to test her new inverter designs.
- Knight:Evolving Reshmaverters - project page.
Results
TetR
Results
Need to port the results described here.
