http://openwetware.org/index.php?title=Endy:FACS_protocols&feed=atom&action=history 2013-05-25T20:40:10Z Revision history for this page on the wiki MediaWiki 1.13.2 http://openwetware.org/index.php?title=Endy:FACS_protocols&diff=148107&oldid=prev 2007-09-05T14:51:56Z

<p>typo</p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 14:51, 5 September 2007</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 6:</td> <td colspan="2" class="diff-lineno">Line 6:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Media==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Media==</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>As <del class="diffchange diffchange-inline">desribed </del>in the standard protocol we often use [[M9_medium/supplemented|Supplemented M9 media]] although we are now switching to using [[Neidhardt_EZ_Rich_Defined|EZ media]] where possible.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>As <ins class="diffchange diffchange-inline">described </ins>in the standard protocol we often use [[M9_medium/supplemented|Supplemented M9 media]] although we are now switching to using [[Neidhardt_EZ_Rich_Defined|EZ media]] where possible.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Bead calibration==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Bead calibration==</div></td></tr> <!-- diff generator: internal 2013-05-25 20:40:10 --> </table>

ClarkeS http://openwetware.org/index.php?title=Endy:FACS_protocols&diff=115702&oldid=prev 2007-05-09T18:36:21Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 18:36, 9 May 2007</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 15:</td> <td colspan="2" class="diff-lineno">Line 15:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Information aggregated by [[Barry Canton]].''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Information aggregated by [[Barry Canton]].''</div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[Category:Protocol]] [[Category:Flow cytometry]]</ins></div></td></tr> <!-- diff generator: internal 2013-05-25 20:40:10 --> </table>

Reshma P. Shetty http://openwetware.org/index.php?title=Endy:FACS_protocols&diff=70436&oldid=prev 2006-09-14T01:24:05Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 01:24, 14 September 2006</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 6:</td> <td colspan="2" class="diff-lineno">Line 6:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Media==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Media==</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>As desribed in the standard protocol we often use [[<del class="diffchange diffchange-inline">M9_media</del>/supplemented|Supplemented M9 media]] although we are now switching to using [[Neidhardt_EZ_Rich_Defined|EZ media]] where possible.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>As desribed in the standard protocol we often use [[<ins class="diffchange diffchange-inline">M9_medium</ins>/supplemented|Supplemented M9 media]] although we are now switching to using [[Neidhardt_EZ_Rich_Defined|EZ media]] where possible.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Bead calibration==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Bead calibration==</div></td></tr> <!-- diff generator: internal 2013-05-25 20:40:10 --> </table>

Barry Canton http://openwetware.org/index.php?title=Endy:FACS_protocols&diff=44906&oldid=prev 2006-06-26T19:07:07Z

<p><span class="autocomment">Data analysis</span></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 19:07, 26 June 2006</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 12:</td> <td colspan="2" class="diff-lineno">Line 12:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Data analysis==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Data analysis==</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>We do all our analysis using [http://www.flowjo.com/ <del class="diffchange diffchange-inline">FloJo</del>] and [http://mathworks.com Matlab].</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>We do all our analysis using [http://www.flowjo.com/ <ins class="diffchange diffchange-inline">FlowJo</ins>] and [http://mathworks.com Matlab].</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Information aggregated by [[Barry Canton]].''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Information aggregated by [[Barry Canton]].''</div></td></tr> <!-- diff generator: internal 2013-05-25 20:40:10 --> </table>

Kchang17 http://openwetware.org/index.php?title=Endy:FACS_protocols&diff=28661&oldid=prev 2006-03-30T18:57:15Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 18:57, 30 March 2006</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 13:</td> <td colspan="2" class="diff-lineno">Line 13:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Data analysis==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Data analysis==</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We do all our analysis using [http://www.flowjo.com/ FloJo] and [http://mathworks.com Matlab].</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We do all our analysis using [http://www.flowjo.com/ FloJo] and [http://mathworks.com Matlab].</div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">''Information aggregated by [[Barry Canton]].''</ins></div></td></tr> <!-- diff generator: internal 2013-05-25 20:40:10 --> </table>

Barry Canton http://openwetware.org/index.php?title=Endy:FACS_protocols&diff=28659&oldid=prev 2006-03-30T18:55:06Z

<p></p> <p><b>New page</b></p><div>==Protocol==<br /> Here is a [[Synthetic Biology:BioBricks/Standard FACS protocol|basic standard protocol]] that we try and use in the Endy and Knight labs. Exact conditions may depend on the strain, induction conditions etc.<br /> <br /> ==Equipment==<br /> When we are doing flow experiments, we normally use a FACScan cytometer, described [http://web.mit.edu/flowcytometry/www/equipment.html here]. Some lab members have experience using the MoFlo machines also. We also have an [[Endy:NPE_flow_cytometer|NPE flow cytometer]] that we use in our lab for some experiments.<br /> <br /> ==Media==<br /> As desribed in the standard protocol we often use [[M9_media/supplemented|Supplemented M9 media]] although we are now switching to using [[Neidhardt_EZ_Rich_Defined|EZ media]] where possible.<br /> <br /> ==Bead calibration==<br /> We calibrate results from day to day using this [[Flow_Calibration|bead protocol]].<br /> <br /> ==Data analysis==<br /> We do all our analysis using [http://www.flowjo.com/ FloJo] and [http://mathworks.com Matlab].</div>

Barry Canton