Endy:FACS protocols

Protocol

Here is a basic standard protocol that we try and use in the Endy and Knight labs. Exact conditions may depend on the strain, induction conditions etc.

Equipment

When we are doing flow experiments, we normally use a FACScan cytometer, described here. Some lab members have experience using the MoFlo machines also. We also have an NPE flow cytometer that we use in our lab for some experiments.

Media

As desribed in the standard protocol we often use Supplemented M9 media although we are now switching to using EZ media where possible.

Bead calibration

We calibrate results from day to day using this bead protocol.

Data analysis

We do all our analysis using FloJo and Matlab.