Endy:Colony PCR protocol
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- Thermo polymerase buffer
- Forward primer
- Reverse primer
- Taq or Vent Polymerase
- Template suspension
- Reaction tubes
- Use a sterile toothpick or pipet tip to resuspend a plated colony in 50 ?l sterile water.
- Store the colony resuspension at 4°C so you can start cultures if necessary (should be OK for a couple days, if you need it to last longer you should use an Index plate.
- Reaction mix
Set up a reaction in reaction tube (1) as follows on ice:
Initial concentration Final concentration Final volume per 10 µl reaction Thermo polymerase buffer 10X 1X 1 µl