Endy:Chemostat: Difference between revisions
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=Setup= | =Setup= | ||
#Wash out chemostat. don't lose the little glass beads. | |||
#autoclave making sure that all tubes are connected. | |||
#setup everything and run media through the device for a little while. this is a good time to calculate the flow rate. | |||
#drop some cells in the reactor and off you go. | |||
=Operation= | =Operation= | ||
Revision as of 15:35, 17 June 2006
This page is for storing information about operation of a bench-scale chemostat designed by Ben Kirkup. It is in development.
Design
- <photo>
- <CAD design> (ask ben if OK)
Setup
- Wash out chemostat. don't lose the little glass beads.
- autoclave making sure that all tubes are connected.
- setup everything and run media through the device for a little while. this is a good time to calculate the flow rate.
- drop some cells in the reactor and off you go.
Operation
Normal Operation
- Setting up the reactor for the run, avoiding contamination, etc.
Troubleshooting/Common problems
Bryan, anything here?
Temperature consistency in water bath
Masterflex tubing in the pump
How did we avoid crushing it?
Contamination
Others?
