Endy:Chassis engineering/VM2.0: Difference between revisions
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#Stability | #Stability | ||
#*Noise | #*Noise | ||
#**Routh-Hurwitz? | |||
#*Response time | #*Response time | ||
#Self-booting/controlled | #Self-booting/controlled | ||
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#Elongating Ribosomes | #Elongating Ribosomes | ||
#etc.</font> | #etc.</font> | ||
===Model analysis notes=== | |||
*A cooperative autogene network can exhibit bistability or monostability depending on parameter values ([http://web.mit.edu/biophysics/sbio/ 7.81]). Does this apply if there is no cooperativity? | |||
Revision as of 07:48, 8 March 2007
VM2.0 regulation design considerations
- Stability
- Noise
- Routh-Hurwitz?
- Response time
- Noise
- Self-booting/controlled
- Ability to turn on or off
- Portability
- Tunable
- Pros and cons of DNA copy number, promoter strength, repressor affinities etc.
- Efficient
- Minimizing levels of repressor needed
- Minimizing consumption of small molecules
What are the metrics for each of the design considerations?
Model
Continuous differential equation model in MATLAB
Species
- T7 RNAP
- Repressor
- Ribosomes
- Repressible T7 promoter
- T7RNAP-promoter complex
- Repressor-promoter complex
- T7 RNAP mRNA
- Repressor mRNA
- Elongating T7 RNAP
- Elongating Ribosomes
- etc.
Model analysis notes
- A cooperative autogene network can exhibit bistability or monostability depending on parameter values (7.81). Does this apply if there is no cooperativity?
