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Stanford New to the lab? Start here (includes safety training, you really must start here) Moving on? End here Lab Evacuation & Rally Points Evacuation plans here Group Meeting Lab Meetings - Time, location, and schedule listed here! Making group meetings more effective Lab Life Responsibilities TASKS: Lab Meetings: room reservation, scheduling of speakers, managing the clock (Ton) 1% agarose (TBD depends how we want to proceed e.g. common/personal stock or make it each time) Sharps (Monica) Preparing Antibiotic Stocks (Jerome) BB enzymes & ligase (Monica) Polymerases (Hi-Fi supermix, Kappa, Pfu Ultra II) (Paul) Strains (Jerome make an inventory and a stock) TAE / TBE / MilliQ (Ton) Pouring Agar plates (Monica, Jerome, and Ton) Preparing competent cells [DH5 alpha Z1] (Jerome and Ton) Hazardous waste removal (no real need yet) Glassware (Elizabeth) Ordering / Inventory of Supplies (Jean/Elizabeth) Pipette Tip Box Recycling [1x per month, put empty boxes in container] (Elizabeth) Autoclaving culture plates (Elizabeth) Washroom User Protocols (Elizabeth) EQUIPMENT Microscopes (Francois) Plate reader (Monica) Centrifuges (Ton) Hot plates / dri-baths / waterbaths (Ton) -20C Freezers, -80C freezers, fridges, cold room Defrosting enzyme blocks (Monica) Defrosting -20C freezers (All) SPACES: Shared Space with Smolke Lab Gel Area (Elizabeth) GETTING STARTED IN THE LAB Training Requirements, Lab Access, and How Things Work EXITING THE LAB Check List for the Graceful Exit Vacation Coverage Sign-ups for vacation coverage Shutdown Journal Club Date: Mondays Time: TBA Location: TBA Suggested Format Journal Club Page SynBERC 2009 Date: February 22-25, 2009 Location: Faculty Club, UC Berkeley Retreat Website Posters Page MIT ERA Archives