Endy:Back Door

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Lab Life Responsibilities)
(Lab Life Responsibilities)
(4 intermediate revisions not shown.)
Line 28: Line 28:
** BB enzymes & ligase ('''Monica''')
** BB enzymes & ligase ('''Monica''')
** Polymerases (Hi-Fi supermix, Kappa, Pfu Ultra II) ('''Paul''')
** Polymerases (Hi-Fi supermix, Kappa, Pfu Ultra II) ('''Paul''')
-
** Strains ('''Jerome & Francois ?''' make an inventory and a stock)
+
** Strains ('''Jerome''' make an inventory and a stock)
** TAE / TBE / MilliQ ('''Ton''')
** TAE / TBE / MilliQ ('''Ton''')
** Pouring [[Agar plates]] ('''Monica, Jerome, and Ton''')
** Pouring [[Agar plates]] ('''Monica, Jerome, and Ton''')
** Preparing competent cells [DH5 alpha Z1] ('''Jerome''' and '''Ton''')
** Preparing competent cells [DH5 alpha Z1] ('''Jerome''' and '''Ton''')
** Hazardous waste removal (no real need yet)
** Hazardous waste removal (no real need yet)
-
** Glassware ('''Elizabeth''')
+
** Glassware ('''Julie''')
-
** Ordering / Inventory of Supplies  ('''Jean/Elizabeth''')
+
** Ordering / Inventory of Supplies  ('''Jean/Julie''')
-
** Pipette Tip Box Recycling [1x per month, put empty boxes in container] ('''Elizabeth''')
+
** Pipette Tip Box Recycling [1x per month, put empty boxes in container] ('''Julie''')
-
** Autoclaving culture plates ('''Elizabeth''')
+
** Autoclaving culture plates ('''Julie''')
-
**[[Washroom_User_Protocols|Washroom User Protocols]] ('''Elizabeth''')
+
**[[Washroom_User_Protocols|Washroom User Protocols]] ('''Julie''')
* EQUIPMENT
* EQUIPMENT
Line 44: Line 44:
** Centrifuges ('''Ton''')
** Centrifuges ('''Ton''')
** Hot plates / dri-baths / waterbaths ('''Ton''')
** Hot plates / dri-baths / waterbaths ('''Ton''')
-
** -20C Freezers, [[Endy:-80C freezers | -80C freezers]], fridges, cold room ('''Francois & Monica?''')
+
** -20C Freezers, [[Endy:-80C freezers | -80C freezers]], fridges, cold room
*** Defrosting enzyme blocks ('''Monica''')
*** Defrosting enzyme blocks ('''Monica''')
-
** System backup ('''Cesar''')
+
*** Defrosting -20C freezers ('''All''')
* SPACES:
* SPACES:
** Shared Space with Smolke Lab
** Shared Space with Smolke Lab
-
** [[Endy:Gel area | Gel Area]] ('''Elizabeth''')
+
** [[Endy:Gel area | Gel Area]] ('''Julie''')
*GETTING STARTED IN THE LAB
*GETTING STARTED IN THE LAB
Line 60: Line 60:
===Vacation Coverage===
===Vacation Coverage===
*[[Vacation_coverage|Sign-ups for vacation coverage]]
*[[Vacation_coverage|Sign-ups for vacation coverage]]
 +
*[[Smolke:Dec shutdown|Shutdown]]
===Journal Club===
===Journal Club===

Revision as of 13:10, 12 March 2013

Home        Lab Members        Research        Notebooks        Publications        Internal        Contact       


Contents

Stanford

New to the lab?

  • Start here (includes safety training, you really must start here)

Moving on?

Lab Evacuation & Rally Points

Group Meeting

Lab Meetings - Time, location, and schedule listed here!

Making group meetings more effective

Lab Life Responsibilities

  • TASKS:
    • Lab Meetings: room reservation, scheduling of speakers, managing the clock (Ton)
    • 1% agarose (TBD depends how we want to proceed e.g. common/personal stock or make it each time)
    • Sharps (Monica)
    • Preparing Antibiotic Stocks (Jerome)
    • BB enzymes & ligase (Monica)
    • Polymerases (Hi-Fi supermix, Kappa, Pfu Ultra II) (Paul)
    • Strains (Jerome make an inventory and a stock)
    • TAE / TBE / MilliQ (Ton)
    • Pouring Agar plates (Monica, Jerome, and Ton)
    • Preparing competent cells [DH5 alpha Z1] (Jerome and Ton)
    • Hazardous waste removal (no real need yet)
    • Glassware (Julie)
    • Ordering / Inventory of Supplies (Jean/Julie)
    • Pipette Tip Box Recycling [1x per month, put empty boxes in container] (Julie)
    • Autoclaving culture plates (Julie)
    • Washroom User Protocols (Julie)
  • EQUIPMENT
    • Microscopes (Francois)
    • Plate reader (Monica)
    • Centrifuges (Ton)
    • Hot plates / dri-baths / waterbaths (Ton)
    • -20C Freezers, -80C freezers, fridges, cold room
      • Defrosting enzyme blocks (Monica)
      • Defrosting -20C freezers (All)
  • SPACES:
    • Shared Space with Smolke Lab
    • Gel Area (Julie)

Vacation Coverage

Journal Club

SynBERC 2009

MIT ERA

Archives

Title text

Personal tools