Endy:Back Door: Difference between revisions
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===New to the lab?=== | ===New to the lab?=== | ||
*Start [[Endy:Starting_Out | here]] (includes safety training, you must start here) | *Start [[Endy:Starting_Out | here]] (includes safety training, '''you really must start here''') | ||
===Moving on?=== | ===Moving on?=== | ||
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===Lab Evacuation & Rally Points=== | ===Lab Evacuation & Rally Points=== | ||
*Evacuation | *Evacuation plans [[Endy:Evacuation | here]] | ||
===Group Meeting=== | ===Group Meeting=== | ||
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** [[Endy:Making 1% Agarose stock | 1% agarose]] ('''TBD''' depends how we want to proceed e.g. common/personal stock or make it each time) | ** [[Endy:Making 1% Agarose stock | 1% agarose]] ('''TBD''' depends how we want to proceed e.g. common/personal stock or make it each time) | ||
** Sharps ('''Monica''') | ** Sharps ('''Monica''') | ||
** [[Endy:Preparing Antibiotic Stocks|Preparing Antibiotic Stocks]] ('''Jerome''') | |||
** [[Endy:Preparing Antibiotic Stocks|Preparing Antibiotic Stocks]] (''' | ** BB enzymes & ligase ('''Monica''') | ||
** | ** Polymerases (Hi-Fi supermix, Kappa, Pfu Ultra II) ('''Paul''') | ||
** | ** Strains ('''Jerome''' make an inventory and a stock) | ||
** Strains ('''Jerome | ** TAE / TBE / MilliQ ('''Ton''') | ||
** TAE / TBE / MilliQ (''' | ** Pouring [[Agar plates]] ('''Monica, Jerome, and Ton''') | ||
** Pouring Agar plates (''' | ** Preparing competent cells [DH5 alpha Z1] ('''Jerome''' and '''Ton''') | ||
** Preparing | |||
** Hazardous waste removal (no real need yet) | ** Hazardous waste removal (no real need yet) | ||
** Glassware (''' | ** Glassware ('''Julie''') | ||
** Ordering / Inventory of Supplies ('''Jean/ | ** Ordering / Inventory of Supplies ('''Jean/Julie''') | ||
** Pipette Tip Box Recycling (''' | ** Pipette Tip Box Recycling [1x per month, put empty boxes in container] ('''Julie''') | ||
** Autoclaving culture plates (''' | ** Autoclaving culture plates ('''Julie''') | ||
**[[Washroom_User_Protocols|Washroom User Protocols]] (''' | **[[Washroom_User_Protocols|Washroom User Protocols]] ('''Julie''') | ||
* EQUIPMENT | * EQUIPMENT | ||
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** Centrifuges ('''Ton''') | ** Centrifuges ('''Ton''') | ||
** Hot plates / dri-baths / waterbaths ('''Ton''') | ** Hot plates / dri-baths / waterbaths ('''Ton''') | ||
** -20C Freezers, [[Endy:-80C freezers | -80C freezers]], fridges, cold room | ** -20C Freezers, [[Endy:-80C freezers | -80C freezers]], fridges, cold room | ||
** | *** Defrosting enzyme blocks ('''Monica''') | ||
* | *** Defrosting -20C freezers ('''All''') | ||
** | |||
* SPACES: | * SPACES: | ||
** Shared Space with Smolke Lab | ** Shared Space with Smolke Lab | ||
** [[Endy:Gel area | Gel Area]] (''' | ** [[Endy:Gel area | Gel Area]] ('''Julie''') | ||
*GETTING STARTED IN THE LAB | *GETTING STARTED IN THE LAB | ||
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**[[Endy:Winding_Up|Check List for the Graceful Exit]] | **[[Endy:Winding_Up|Check List for the Graceful Exit]] | ||
===Vacation Coverage | ===Vacation Coverage=== | ||
*[[Vacation_coverage|Sign-ups for vacation coverage]] | *[[Vacation_coverage|Sign-ups for vacation coverage]] | ||
*[[Smolke:Dec shutdown|Shutdown]] | |||
===Journal Club=== | ===Journal Club=== | ||
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[[MIT Era|Archives]] | [[MIT Era|Archives]] | ||
[[Image:OWW Logo.png|150px |link=http://www.example.org |alt=Alt text|Title text]] |
Revision as of 11:10, 12 March 2013
StanfordNew to the lab?
Moving on?
Lab Evacuation & Rally Points
Group MeetingLab Meetings - Time, location, and schedule listed here! Making group meetings more effective Lab Life Responsibilities
Vacation CoverageJournal Club
SynBERC 2009
MIT ERA |