Endy:Back Door
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** [[Endy:Making 1% Agarose stock | 1% agarose]] ('''TBD''' depends how we want to proceed e.g. common/personal stock or make it each time) | ** [[Endy:Making 1% Agarose stock | 1% agarose]] ('''TBD''' depends how we want to proceed e.g. common/personal stock or make it each time) | ||
** Sharps ('''Monica''') | ** Sharps ('''Monica''') | ||
| - | + | ** [[Endy:Preparing Antibiotic Stocks|Preparing Antibiotic Stocks]] ('''Jerome''') | |
| - | ** [[Endy:Preparing Antibiotic Stocks|Preparing Antibiotic Stocks]] (''' | + | ** BB enzymes & ligase ('''Monica''') |
| - | ** | + | ** Polymerases (Hi-Fi supermix, Kappa, Pfu Ultra II) ('''Paul''') |
| - | ** | + | |
** Strains ('''Jerome & Francois ?''' make an inventory and a stock) | ** Strains ('''Jerome & Francois ?''' make an inventory and a stock) | ||
| - | ** TAE / TBE / MilliQ (''' | + | ** TAE / TBE / MilliQ ('''Ton''') |
| - | ** Pouring [[Agar plates]] ( | + | ** Pouring [[Agar plates]] ('''Monica, Jerome, and Ton''') |
| - | ** Preparing competent cells | + | ** Preparing competent cells [DH5 alpha Z1] ('''Jerome''' and '''Ton''') |
** Hazardous waste removal (no real need yet) | ** Hazardous waste removal (no real need yet) | ||
** Glassware ('''Elizabeth''') | ** Glassware ('''Elizabeth''') | ||
** Ordering / Inventory of Supplies ('''Jean/Elizabeth''') | ** Ordering / Inventory of Supplies ('''Jean/Elizabeth''') | ||
| - | ** Pipette Tip Box Recycling ('''Elizabeth''') | + | ** Pipette Tip Box Recycling [1x per month, put empty boxes in container] ('''Elizabeth''') |
** Autoclaving culture plates ('''Elizabeth''') | ** Autoclaving culture plates ('''Elizabeth''') | ||
**[[Washroom_User_Protocols|Washroom User Protocols]] ('''Elizabeth''') | **[[Washroom_User_Protocols|Washroom User Protocols]] ('''Elizabeth''') | ||
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** Hot plates / dri-baths / waterbaths ('''Ton''') | ** Hot plates / dri-baths / waterbaths ('''Ton''') | ||
** -20C Freezers, [[Endy:-80C freezers | -80C freezers]], fridges, cold room ('''Francois & Monica?''') | ** -20C Freezers, [[Endy:-80C freezers | -80C freezers]], fridges, cold room ('''Francois & Monica?''') | ||
| + | *** Defrosting enzyme blocks ('''Monica''') | ||
** System backup ('''Cesar''') | ** System backup ('''Cesar''') | ||
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* SPACES: | * SPACES: | ||
Revision as of 14:10, 30 June 2011
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